Antitubercular constituents of Valeriana laxiflora

Jian Qiao Gu, Yuehong Wang, Scott G. Franzblau, Gloria Montenegro, Danzhou Yang, Barbara N. Timmermann

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

Antitubercular bioassay-guided fractionation of the n-hexane-and CH 2Cl2-soluble extracts of the above-ground biomass and roots of Valeriana laxiflora led to the isolation of a new iridolactone, (4R,5R,7S,8S,9S)-7-hydroxy-8-hydroxymethyl-4-methyl-perhydrocyclopenta[c] pyran-1-one (1), and a new lignan, (+)-1-hydroxy-2,6-bis-epi-pinoresinol (2), along with eleven known compounds, betulin (3), betulinic acid (4), 5,7-dihydroxy-3,6,4′-trimethoxyflavone (5), 23-hydroxyursolic acid (6), oleanolic acid (7), tricin (8), ursolic acid (9), ferulic acid, (+)-1-hydroxypinoresinol, prinsepiol, and 5,7,3′-trihydroxy-4′- methoxyflavone. The structures of compounds 1 and 2 were elucidated on the basis of spectroscopic evidence. The absolute stereochemistry of 1 was determined by chemical transformations and Mosher ester procedures. In a microplate alamar blue assay against Mycobacterium tuberculosis, compounds 2-9 exhibited minimum inhibitory concentrations (MIC) of 15.5 - 127 μg/mL, while the other isolates were regarded as inactive (MIC > 128 μg/mL). In addition, all the isolates were tested for cytotoxicity against African green monkey Vero cells in order to evaluate their selectivity potential.

Original languageEnglish (US)
Pages (from-to)509-514
Number of pages6
JournalPlanta Medica
Volume70
Issue number6
DOIs
StatePublished - Jun 1 2004

Keywords

  • Antimycobacterial activity
  • Cytotoxicity
  • Iridolactone
  • Lignan
  • Triterpene
  • Valeriana laxiflora
  • Valerianaceae

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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  • Cite this

    Gu, J. Q., Wang, Y., Franzblau, S. G., Montenegro, G., Yang, D., & Timmermann, B. N. (2004). Antitubercular constituents of Valeriana laxiflora. Planta Medica, 70(6), 509-514. https://doi.org/10.1055/s-2004-827149