Apical spectrin is essential for epithelial morphogenesis but not apicobasal polarity in Drosophila

Daniela C Zarnescu, Graham H. Thomas

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Changes in cell shape and position drive morphogenesis in epithelia and depend on the polarized nature of its constituent cells. The spectrin-based membrane skeleton is thought to be a key player in the establishment and/or maintenance of cell shape and polarity. We report that apical β(Heavy)- Spectrin (β(H)), a terminal web protein that is also associated with the zonula adherens, is essential for normal epithelial morphogenesis of the Drosophila follicle cell epithelium during oogenesis. Elimination of β(H) by the karst mutation prevents apical constriction of the follicle cells during mid-oogenesis, and is accompanied by a gross breakup of the zonula adherens. We also report that the integrity of the migratory border cell cluster, a group of anterior follicle cells that delaminates from the follicle epithelium, is disrupted. Elimination of β(H) prevents the stable recruitment of α-spectrin to the apical domain, but does not result in a loss of apicobasal polarity, as would be predicted from current models describing the role of spectrin in the establishment of cell polarity. These results demonstrate a direct role for apical (αβ(H)2-spectrin in epithelial morphogenesis driven by apical contraction, and suggest that apical and basolateral spectrin do not play identical roles in the generation of apicobasal polarity.

Original languageEnglish (US)
Pages (from-to)1075-1086
Number of pages12
JournalJournal of Cell Biology
Volume146
Issue number5
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Spectrin
Morphogenesis
Drosophila
Adherens Junctions
Oogenesis
Cell Polarity
Epithelium
Cell Shape
Skeleton
Constriction
Maintenance
Mutation
Membranes
Proteins

Keywords

  • Cell polarity
  • Morphogenesis
  • Oogenesis
  • Spectrin
  • Zonula adherens

ASJC Scopus subject areas

  • Cell Biology

Cite this

Apical spectrin is essential for epithelial morphogenesis but not apicobasal polarity in Drosophila. / Zarnescu, Daniela C; Thomas, Graham H.

In: Journal of Cell Biology, Vol. 146, No. 5, 1999, p. 1075-1086.

Research output: Contribution to journalArticle

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