Apolipoprotein E-rich HDL binding to liver plasma membranes in copper-deficient rats

C. A. Hassel, T. P. Carr, J. A. Marchello, John A Marchello

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Abstract

Copper deficiency in rat raises plasma cholesterol concentration while reducing liver cholesterol concentration. One consequence of this cholesterol redistribution is the accumulation of a large high-density lipoprotein (HDL) particle rich in apolipoprotein E (apo E). The purpose of this study was to determine, using an in vitro binding assay, if the interaction of apo E-rich HDL with hepatic lipoprotein binding sites may be affected by copper deficiency. Male Sprague-Dawley rats were divided into two dietary treatments (copper-deficient and -adequate) and placed on a dietary regimen for 8 weeks. Subsequent to exsanguination, hepatic plasma membranes were prepared and apo E-rich HDL was isolated from rats of each treatment by ultracentrifugation, agarose column chromatography, and heparin-Sepharose affinity chromatography. Total binding and experimentally derived specific binding of 125I-apo E-rich HDL to hepatic plasma membranes indicated greater binding when lipoproteins and membranes from copper-deficient animals were used in the assay compared to controls. Scatchard analysis of specific binding data indicated that equilibrium binding affinity (K(d)) was also affected by copper deficiency. The hepatic binding sites recognizing apo E-rich HDL were not affected by EDTA or pronase, or relatively high capacity, and recognized a variety of other rat lipoproteins.

Original languageEnglish (US)
Pages (from-to)296-308
Number of pages13
JournalProceedings of the Society for Experimental Biology and Medicine
Volume187
Issue number3
StatePublished - 1988

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Apolipoproteins E
HDL Lipoproteins
Cell membranes
Liver
Rats
Copper
Cell Membrane
Lipoproteins
Agarose Chromatography
Cholesterol
Assays
Binding Sites
Exsanguination
Affinity chromatography
Pronase
Column chromatography
Ultracentrifugation
Affinity Chromatography
Edetic Acid
Sepharose

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Apolipoprotein E-rich HDL binding to liver plasma membranes in copper-deficient rats. / Hassel, C. A.; Carr, T. P.; Marchello, J. A.; Marchello, John A.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 187, No. 3, 1988, p. 296-308.

Research output: Contribution to journalArticle

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