Apoptosis of JHMV-specific CTL in the CNS in the absence of CD4+ T cells

S. A. Stohlman, C. C. Bergmann, D. J. Cua, Mark T Lin, S. Ho, W. Wei, D. R. Hinton

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6 Scopus citations

Abstract

The role of CD4+ T cells in altering the activity of cytotoxic T lymphocytes (CTL) during infection of the central nervous system (CNS) by the neuroptropic JHMV strain of mouse hepatitis virus was examined. Adoptive transfer of in vitro activated CTL into CD4-depleted and control recipients showed that CTL were not effective in reducing JHMV replication within the CNS. The distribution of CD4+ and CD8+ T cells within the CNS during JHMV infection showed that the CD4+ T cells remained in perivascular and subarachnoid spaces and few entered the parenchyma. By contrast approximately half of the CD8+ T cells entered the parenchyma. In CD4-depleted mice the trafficking of CD8+ T cells was not inhibited; however, the majority of the cells were found to be apoptotic. These data suggested that CD4+ T cells were not required for CTL induction but were required for the maintenance of CTL viability. The limited role of CD4+ T cells in CTL induction was confirmed by comparison of CTL activity from CD4-depleted and control mice.

Original languageEnglish (US)
Pages (from-to)425-430
Number of pages6
JournalAdvances in Experimental Medicine and Biology
Volume440
Publication statusPublished - 1998
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Stohlman, S. A., Bergmann, C. C., Cua, D. J., Lin, M. T., Ho, S., Wei, W., & Hinton, D. R. (1998). Apoptosis of JHMV-specific CTL in the CNS in the absence of CD4+ T cells. Advances in Experimental Medicine and Biology, 440, 425-430.