Apoptotic, necrotic, or fused tumor cells: An equivalent source of antigen for dendritic cell loading

Nicolas Larmonier, Delphine Mérino, Alexandra Nicolas, Dominique Cathelin, Angélique Besson, Andrew Bateman, Eric Solary, François Martin, Emmanuel Katsanis, Bernard Bonnotte

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The identification of the most efficient strategy for tumor antigen loading of dendritic cells (DCs) remains a challenge in cancer immunotherapy protocols. Autologous dead tumor cells have been demonstrated to constitute an acceptable source of multiple tumor-associated antigens (TAA) to pulse DCs. However the optimal approach for inducing cell death that would lead to effective endocytosis and activation of DCs remains controversial. In this study we have induced and defined 3 distinct mechanisms of tumor cell death (apoptosis, necrosis and fusion-mediated cell death), and investigated their differential effects on DCs. Bone marrow-derived DCs demonstrated comparable uptake of primary apoptotic, necrotic, or fused dead tumor cells. Furthermore, the distinct modes of cancer cell death had analogous potential in activating the transcription factors NF-κB and STAT1 and in maturing DCs, resulting in an equally effective stimulation of immune T cells. The current study therefore provides further informations on the use of dead whole tumor cells as antigen sources for effective active anti-cancer immunotherapy.

Original languageEnglish (US)
Pages (from-to)1513-1524
Number of pages12
JournalApoptosis
Volume11
Issue number9
DOIs
StatePublished - Sep 2006

Fingerprint

Dendritic Cells
Tumors
Cells
Cell death
Antigens
Cell Death
Neoplasms
Neoplasm Antigens
Immunotherapy
Activating Transcription Factors
T-cells
Cell Fusion
Endocytosis
Bone
Fusion reactions
Chemical activation
Apoptosis
Necrosis
Bone Marrow
T-Lymphocytes

Keywords

  • Antigen presentation
  • Apoptosis
  • Dendritic cells
  • Fusion
  • NF-κB
  • Tumor cell death

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Larmonier, N., Mérino, D., Nicolas, A., Cathelin, D., Besson, A., Bateman, A., ... Bonnotte, B. (2006). Apoptotic, necrotic, or fused tumor cells: An equivalent source of antigen for dendritic cell loading. Apoptosis, 11(9), 1513-1524. https://doi.org/10.1007/s10495-006-8765-0

Apoptotic, necrotic, or fused tumor cells : An equivalent source of antigen for dendritic cell loading. / Larmonier, Nicolas; Mérino, Delphine; Nicolas, Alexandra; Cathelin, Dominique; Besson, Angélique; Bateman, Andrew; Solary, Eric; Martin, François; Katsanis, Emmanuel; Bonnotte, Bernard.

In: Apoptosis, Vol. 11, No. 9, 09.2006, p. 1513-1524.

Research output: Contribution to journalArticle

Larmonier, N, Mérino, D, Nicolas, A, Cathelin, D, Besson, A, Bateman, A, Solary, E, Martin, F, Katsanis, E & Bonnotte, B 2006, 'Apoptotic, necrotic, or fused tumor cells: An equivalent source of antigen for dendritic cell loading', Apoptosis, vol. 11, no. 9, pp. 1513-1524. https://doi.org/10.1007/s10495-006-8765-0
Larmonier, Nicolas ; Mérino, Delphine ; Nicolas, Alexandra ; Cathelin, Dominique ; Besson, Angélique ; Bateman, Andrew ; Solary, Eric ; Martin, François ; Katsanis, Emmanuel ; Bonnotte, Bernard. / Apoptotic, necrotic, or fused tumor cells : An equivalent source of antigen for dendritic cell loading. In: Apoptosis. 2006 ; Vol. 11, No. 9. pp. 1513-1524.
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