Abstract
The identification of the most efficient strategy for tumor antigen loading of dendritic cells (DCs) remains a challenge in cancer immunotherapy protocols. Autologous dead tumor cells have been demonstrated to constitute an acceptable source of multiple tumor-associated antigens (TAA) to pulse DCs. However the optimal approach for inducing cell death that would lead to effective endocytosis and activation of DCs remains controversial. In this study we have induced and defined 3 distinct mechanisms of tumor cell death (apoptosis, necrosis and fusion-mediated cell death), and investigated their differential effects on DCs. Bone marrow-derived DCs demonstrated comparable uptake of primary apoptotic, necrotic, or fused dead tumor cells. Furthermore, the distinct modes of cancer cell death had analogous potential in activating the transcription factors NF-κB and STAT1 and in maturing DCs, resulting in an equally effective stimulation of immune T cells. The current study therefore provides further informations on the use of dead whole tumor cells as antigen sources for effective active anti-cancer immunotherapy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1513-1524 |
| Number of pages | 12 |
| Journal | Apoptosis |
| Volume | 11 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2006 |
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Keywords
- Antigen presentation
- Apoptosis
- Dendritic cells
- Fusion
- NF-κB
- Tumor cell death
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
Cite this
Apoptotic, necrotic, or fused tumor cells : An equivalent source of antigen for dendritic cell loading. / Larmonier, Nicolas; Mérino, Delphine; Nicolas, Alexandra; Cathelin, Dominique; Besson, Angélique; Bateman, Andrew; Solary, Eric; Martin, François; Katsanis, Emmanuel; Bonnotte, Bernard.
In: Apoptosis, Vol. 11, No. 9, 09.2006, p. 1513-1524.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apoptotic, necrotic, or fused tumor cells
T2 - An equivalent source of antigen for dendritic cell loading
AU - Larmonier, Nicolas
AU - Mérino, Delphine
AU - Nicolas, Alexandra
AU - Cathelin, Dominique
AU - Besson, Angélique
AU - Bateman, Andrew
AU - Solary, Eric
AU - Martin, François
AU - Katsanis, Emmanuel
AU - Bonnotte, Bernard
PY - 2006/9
Y1 - 2006/9
N2 - The identification of the most efficient strategy for tumor antigen loading of dendritic cells (DCs) remains a challenge in cancer immunotherapy protocols. Autologous dead tumor cells have been demonstrated to constitute an acceptable source of multiple tumor-associated antigens (TAA) to pulse DCs. However the optimal approach for inducing cell death that would lead to effective endocytosis and activation of DCs remains controversial. In this study we have induced and defined 3 distinct mechanisms of tumor cell death (apoptosis, necrosis and fusion-mediated cell death), and investigated their differential effects on DCs. Bone marrow-derived DCs demonstrated comparable uptake of primary apoptotic, necrotic, or fused dead tumor cells. Furthermore, the distinct modes of cancer cell death had analogous potential in activating the transcription factors NF-κB and STAT1 and in maturing DCs, resulting in an equally effective stimulation of immune T cells. The current study therefore provides further informations on the use of dead whole tumor cells as antigen sources for effective active anti-cancer immunotherapy.
AB - The identification of the most efficient strategy for tumor antigen loading of dendritic cells (DCs) remains a challenge in cancer immunotherapy protocols. Autologous dead tumor cells have been demonstrated to constitute an acceptable source of multiple tumor-associated antigens (TAA) to pulse DCs. However the optimal approach for inducing cell death that would lead to effective endocytosis and activation of DCs remains controversial. In this study we have induced and defined 3 distinct mechanisms of tumor cell death (apoptosis, necrosis and fusion-mediated cell death), and investigated their differential effects on DCs. Bone marrow-derived DCs demonstrated comparable uptake of primary apoptotic, necrotic, or fused dead tumor cells. Furthermore, the distinct modes of cancer cell death had analogous potential in activating the transcription factors NF-κB and STAT1 and in maturing DCs, resulting in an equally effective stimulation of immune T cells. The current study therefore provides further informations on the use of dead whole tumor cells as antigen sources for effective active anti-cancer immunotherapy.
KW - Antigen presentation
KW - Apoptosis
KW - Dendritic cells
KW - Fusion
KW - NF-κB
KW - Tumor cell death
UR - http://www.scopus.com/inward/record.url?scp=33747424377&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33747424377&partnerID=8YFLogxK
U2 - 10.1007/s10495-006-8765-0
DO - 10.1007/s10495-006-8765-0
M3 - Article
C2 - 16738802
AN - SCOPUS:33747424377
VL - 11
SP - 1513
EP - 1524
JO - Apoptosis : an international journal on programmed cell death
JF - Apoptosis : an international journal on programmed cell death
SN - 1360-8185
IS - 9
ER -