Appearance of langerhans cells in the epidermis of Tgfb1-/- SCID mice: Paracrine and autocrine effects of transforming growth factor-β1 and -β21

R. M. Thomas, D. V. Belsito, C. Huang, L. Z. Chen, I. Ormsby, W. J. Simmons, P. Cowin, J. Shaw, Thomas C Doetschman, G. J. Thorbecke

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A striking immunologic abnormality of normal and SCID Tgfb1-/- mice is the total absence of Langerhans cells in their epidermis. Here we show that transfer of Tgfb1+/- SCID bone marrow causes, within a few weeks, the appearance of Langerhans cells in the epidermis of γ-irradiated and unirradiated Tgfb1-/- SCID recipients. In addition, local injection of 2 × 105 latent transforming growth factor-β1 cDNA-transduced cloned CD4+ T lymphocytes causes the appearance of Langerhans cells in the ear epidermis of Tgfb1-/- SCID mice. This effect is enhanced by antigen-specific activation of these T cells. Injection of recombinant active transforming growth factor-β2 into the ear of Tgfb1-/- SCID mice also results in the migration of Langerhans cells into the epidermis locally, but no epidermal Langerhans cells are seen after systemic injections of transforming growth factor-β2. Our results suggest that transforming growth factor-β can act in paracrine as well as autocrine fashion to induce the differentiation of precursors into Langerhans cells. Furthermore, these results indicate that the relative roles of different transforming growth factor-β isoforms in vivo may be influenced by their local availability and/or the regulation of their conversion from latent into active form.

Original languageEnglish (US)
Pages (from-to)1574-1580
Number of pages7
JournalJournal of Investigative Dermatology
Volume117
Issue number6
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

SCID Mice
Langerhans Cells
Transforming Growth Factors
Epidermis
T-cells
Injections
Ear
CD27 Antigens
Protein Isoforms
Bone
Complementary DNA
Chemical activation
Availability
Antigens
Bone Marrow
T-Lymphocytes

Keywords

  • Antigen presentation
  • Cytokines
  • Knockout
  • Transgenic

ASJC Scopus subject areas

  • Dermatology

Cite this

Appearance of langerhans cells in the epidermis of Tgfb1-/- SCID mice : Paracrine and autocrine effects of transforming growth factor-β1 and -β21. / Thomas, R. M.; Belsito, D. V.; Huang, C.; Chen, L. Z.; Ormsby, I.; Simmons, W. J.; Cowin, P.; Shaw, J.; Doetschman, Thomas C; Thorbecke, G. J.

In: Journal of Investigative Dermatology, Vol. 117, No. 6, 2001, p. 1574-1580.

Research output: Contribution to journalArticle

Thomas, R. M. ; Belsito, D. V. ; Huang, C. ; Chen, L. Z. ; Ormsby, I. ; Simmons, W. J. ; Cowin, P. ; Shaw, J. ; Doetschman, Thomas C ; Thorbecke, G. J. / Appearance of langerhans cells in the epidermis of Tgfb1-/- SCID mice : Paracrine and autocrine effects of transforming growth factor-β1 and -β21. In: Journal of Investigative Dermatology. 2001 ; Vol. 117, No. 6. pp. 1574-1580.
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