Application of gene probes as diagnostic tools for White Spot Baculovirus (WSBV) of penaeid shrimp

Stephanie Durand, Donald V Lightner, Linda M. Nunan, Rita M. Redman, Jocelyne Mari, Jean Robert Bonami

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Since 1993, similar baculoviruses, which cause high mortalities in penaeid shrimp, have been reported from China, Japan, Korea, Thailand and Taiwan. All these baculovirus-caused diseases are characterized by the presence of white spots in the cuticle. To isolate the agent of the disease referred to as White Spot Syndrome (WSS) and White Spot Baculovirus (WSBV), in this paper, Penaeus vannamei and P. stylirostris were experimentally inoculated with homogenates of infected Penaeus monodon from Thailand. In transmission electron microscopy (TEM), the enveloped WSBV virions have a size of about 350 nm long and 130 nm wide. The nucleocapsids range from 300 to 420 nm in length and 70 to 95 nm in diameter and show a superficially segmented appearance. Also present are unique particles, which have not been observed before, measuring 400 nm in length and 120 nm in width. These particles are larger than most of the usual nucleocapsids and have a cross- batched superficial appearance. After nucleic acid extraction, EcoRI-digested fragments of the WSBV genome were cloned. Four of these fragments were characterized and used as non-radioactive probes lableled with DIG-11-dUTP. By in situ hybridization, the probes hybridized with material located in nuclei of all WSBV-infected tissues. Previously reported target tissues, such as connective tissue, epithelial tissue and also hemocytes, clearly showed positive hybridization with the probes. In addition, some light infection was revealed in the muscle and nervous tissue.

Original languageEnglish (US)
Pages (from-to)59-66
Number of pages8
JournalDiseases of Aquatic Organisms
Volume27
Issue number1
StatePublished - Oct 17 1996

Fingerprint

White spot syndrome virus
Penaeidae
probes (equipment)
probe
nucleocapsid
gene
Baculoviridae
Thailand
genes
white spot syndrome
Litopenaeus stylirostris
Penaeus monodon
Litopenaeus vannamei
hemocytes
virion
connective tissues
in situ hybridization
nucleic acids
Korean Peninsula
transmission electron microscopy

Keywords

  • Gene probes
  • In situ hybridization
  • Non-occluded baculovirus
  • Penaeid shrimp
  • White Spot Syndrome

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

Cite this

Application of gene probes as diagnostic tools for White Spot Baculovirus (WSBV) of penaeid shrimp. / Durand, Stephanie; Lightner, Donald V; Nunan, Linda M.; Redman, Rita M.; Mari, Jocelyne; Bonami, Jean Robert.

In: Diseases of Aquatic Organisms, Vol. 27, No. 1, 17.10.1996, p. 59-66.

Research output: Contribution to journalArticle

Durand, Stephanie ; Lightner, Donald V ; Nunan, Linda M. ; Redman, Rita M. ; Mari, Jocelyne ; Bonami, Jean Robert. / Application of gene probes as diagnostic tools for White Spot Baculovirus (WSBV) of penaeid shrimp. In: Diseases of Aquatic Organisms. 1996 ; Vol. 27, No. 1. pp. 59-66.
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