Application of gene probes to determine target organs of a penaeid shrimp baculovirus using in situ hybridization

Linda D. Bruce, Rita M. Redman, Donald V. Lightner

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Gene probes developed to the penaeid shrimp virus, Baculovirus penaei (BP), were utilized to determine the target organs infected by BP. BP-DNA fragments were nonradioactively labelled with digoxigenin and used to probe paraffin-embedded, fixed tissue sections using an in situ hybridization assay system. BP-infected tissue sections were microscopically examined for a blue-purple probe precipitate indicative of a positive reaction for BP. Particular attention was given to the reproductive tissues of mature shrimp, to determine if vertical transmission might occur. BP positive cells were located only in the hepatopancreatic tubule epithelium and in the midgut mucosa. These findings indicate that BP is a gut-infecting baculovirus that is transmitted horizontally by the per os route and not by true vertical transmission routes.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
JournalAquaculture
Volume120
Issue number1-2
DOIs
StatePublished - Feb 15 1994

ASJC Scopus subject areas

  • Aquatic Science

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