APPLICATION OF GENE PROBES TO POLIOVIRUS AND HEPATITIS A VIRUS DETECTION IN WATER AND SHELLFISH.

Aaron B. Margolin, Michael S. Bitrick, Ricardo DeLeon, Charles P. Gerba

Research output: Contribution to journalConference article

1 Scopus citations

Abstract

The authors have developed cDNA probes for poliovirus type 1 and hepatitis A virus labeled with both **3**2P dATP and dCTP which are reported to be capable of detecting as little as one femtogram of nucleic acid, one tissue culture infectious dose of poliovirus type 1 or one radio-immuno-focus unit of hepatitis A virus. Results are obtained within 72 hours as cell culture is not necessary. The authors have successfully applied this method to the detection of enteroviruses in both water and shellfish concentrates.

Original languageEnglish (US)
Pages (from-to)1746-1751
Number of pages6
JournalOceans Conference Record (IEEE)
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Oceanography

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