APPLICATION OF GENE PROBES TO POLIOVIRUS AND HEPATITIS A VIRUS DETECTION IN WATER AND SHELLFISH.

Aaron B. Margolin, Michael S. Bitrick, Ricardo DeLeon, Charles P Gerba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The authors have developed cDNA probes for poliovirus type 1 and hepatitis A virus labeled with both **3**2P dATP and dCTP which are reported to be capable of detecting as little as one femtogram of nucleic acid, one tissue culture infectious dose of poliovirus type 1 or one radio-immuno-focus unit of hepatitis A virus. Results are obtained within 72 hours as cell culture is not necessary. The authors have successfully applied this method to the detection of enteroviruses in both water and shellfish concentrates.

Original languageEnglish (US)
Title of host publicationOceans Conference Record (IEEE)
PublisherIEEE
Pages1746-1751
Number of pages6
StatePublished - 1987

Fingerprint

hepatitis
shellfish
virus
probe
gene
nucleic acid
radio
water
detection
dose
method
enterovirus
tissue

ASJC Scopus subject areas

  • Oceanography

Cite this

Margolin, A. B., Bitrick, M. S., DeLeon, R., & Gerba, C. P. (1987). APPLICATION OF GENE PROBES TO POLIOVIRUS AND HEPATITIS A VIRUS DETECTION IN WATER AND SHELLFISH. In Oceans Conference Record (IEEE) (pp. 1746-1751). IEEE.

APPLICATION OF GENE PROBES TO POLIOVIRUS AND HEPATITIS A VIRUS DETECTION IN WATER AND SHELLFISH. / Margolin, Aaron B.; Bitrick, Michael S.; DeLeon, Ricardo; Gerba, Charles P.

Oceans Conference Record (IEEE). IEEE, 1987. p. 1746-1751.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Margolin, AB, Bitrick, MS, DeLeon, R & Gerba, CP 1987, APPLICATION OF GENE PROBES TO POLIOVIRUS AND HEPATITIS A VIRUS DETECTION IN WATER AND SHELLFISH. in Oceans Conference Record (IEEE). IEEE, pp. 1746-1751.
Margolin AB, Bitrick MS, DeLeon R, Gerba CP. APPLICATION OF GENE PROBES TO POLIOVIRUS AND HEPATITIS A VIRUS DETECTION IN WATER AND SHELLFISH. In Oceans Conference Record (IEEE). IEEE. 1987. p. 1746-1751
Margolin, Aaron B. ; Bitrick, Michael S. ; DeLeon, Ricardo ; Gerba, Charles P. / APPLICATION OF GENE PROBES TO POLIOVIRUS AND HEPATITIS A VIRUS DETECTION IN WATER AND SHELLFISH. Oceans Conference Record (IEEE). IEEE, 1987. pp. 1746-1751
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abstract = "The authors have developed cDNA probes for poliovirus type 1 and hepatitis A virus labeled with both **3**2P dATP and dCTP which are reported to be capable of detecting as little as one femtogram of nucleic acid, one tissue culture infectious dose of poliovirus type 1 or one radio-immuno-focus unit of hepatitis A virus. Results are obtained within 72 hours as cell culture is not necessary. The authors have successfully applied this method to the detection of enteroviruses in both water and shellfish concentrates.",
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