Application of molecular diagnostic methods to penaeid shrimp diseases: Advances of the past 10 years for control of viral diseases in farmed shrimp

Donald V Lightner, B. T. Poulos, Feng-Jyu Tang-Nelson, Carlos R Pantoja-Morales, L. M. Nunan, S. A. Navarro, R. M. Redman, L. L. Mohney

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The most important diseases of farmed penaeid shrimp have infectious aetiologies. Among these are diseases with viral, rickettsial, bacterial, fungal and parasitic aetiologies. Diagnostic methods for these pathogens include the traditional methods of gross pathology, histopathology, classical microbiology, animal bioassay, antibody-based methods, and molecular methods using DNA probes and DNA amplification. While methods using clinical chemistry and tissue culture are standard methods in veterinary and human diagnostic laboratories, the former has not been routinely applied to the diagnosis of penaeid shrimp diseases and the latter has yet to be developed, despite considerable research and development efforts that have spanned the past 40 years. No continuous shrimp cell lines, or lines from other crustaceans, have been developed. Hence, when molecular methods began to be routinely applied to the diagnosis of infectious diseases in humans and domestic animals in the mid- to late 1980s, the technology was applied to the diagnosis of certain important diseases of penaeid shrimp for which only classical diagnostic methods were previously available. A DNA hybridization assay for the parvovirus IHHNV was the first molecular test developed for a shrimp disease. This was followed within a year by the first PCR test for MBV, an important baculovirus disease of shrimp. Today, shrimp disease diagnostic laboratories routinely use molecular tests for diagnostic and surveillance purposes for most of the important penaeid shrimp diseases.

Original languageEnglish (US)
Pages (from-to)117-122
Number of pages6
JournalDevelopments in Biologicals
Volume126
StatePublished - 2006

Fingerprint

Penaeidae
Molecular Pathology
Virus Diseases
DNA
Densovirinae
Animals
Parvovirus
Clinical Chemistry
Microbiology
Baculoviridae
Tissue culture
Domestic Animals
DNA Probes
Bioassay
Biological Assay
Pathology
Pathogens
Communicable Diseases
Antibodies
Amplification

Keywords

  • PCR
  • Penaeus
  • RT-PCR
  • Shrimp viruses

ASJC Scopus subject areas

  • Drug Discovery
  • Immunology and Microbiology(all)
  • Public Health, Environmental and Occupational Health

Cite this

Application of molecular diagnostic methods to penaeid shrimp diseases : Advances of the past 10 years for control of viral diseases in farmed shrimp. / Lightner, Donald V; Poulos, B. T.; Tang-Nelson, Feng-Jyu; Pantoja-Morales, Carlos R; Nunan, L. M.; Navarro, S. A.; Redman, R. M.; Mohney, L. L.

In: Developments in Biologicals, Vol. 126, 2006, p. 117-122.

Research output: Contribution to journalArticle

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