Application of pooled monoclonal antibodies for 1-hr detection of respiratory syncytial virus antigen in clinical specimens

Linda L. Minnich, Ziad M Shehab, C. George Ray

Research output: Contribution to journalArticle

Abstract

A fluorescein isothiocyanate-conjugated pool of monoclonal antibodies (MoAb) to respiratory syncytial virus (RSV) was prospectively evaluated for its utility as a direct, 1-hr test for the diagnosis of RSV infection. Direct nasopharyngeal swab smears collected from 109 infants and children with acute respiratory illnesses were studied and compared with results obtained by indirect immunofluorescence using bovine polyclonal anti-RSV antibody on eluted cells derived from pooled nasopharyngeal and throat swab specimens (a 2.5-3 hr procedure), and culture. The MoAb-direct smear method was at least 86%-89% sensitive and 95%-100% specific compared with either of the other procedures. Additional prospective evaluations, as well as retrospective studies on a selected bank of slides stored from the preceding year, established that this MoAb could also be used with confidence in testing where direct smears are not employed.

Original languageEnglish (US)
Pages (from-to)137-141
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume7
Issue number2
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Respiratory Syncytial Viruses
Monoclonal Antibodies
Antigens
Respiratory Syncytial Virus Infections
Indirect Fluorescent Antibody Technique
Pharynx
Fluorescein
Retrospective Studies
Prospective Studies
Antibodies

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Application of pooled monoclonal antibodies for 1-hr detection of respiratory syncytial virus antigen in clinical specimens. / Minnich, Linda L.; Shehab, Ziad M; Ray, C. George.

In: Diagnostic Microbiology and Infectious Disease, Vol. 7, No. 2, 1987, p. 137-141.

Research output: Contribution to journalArticle

@article{8c285887cf474419b3f1be374679170d,
title = "Application of pooled monoclonal antibodies for 1-hr detection of respiratory syncytial virus antigen in clinical specimens",
abstract = "A fluorescein isothiocyanate-conjugated pool of monoclonal antibodies (MoAb) to respiratory syncytial virus (RSV) was prospectively evaluated for its utility as a direct, 1-hr test for the diagnosis of RSV infection. Direct nasopharyngeal swab smears collected from 109 infants and children with acute respiratory illnesses were studied and compared with results obtained by indirect immunofluorescence using bovine polyclonal anti-RSV antibody on eluted cells derived from pooled nasopharyngeal and throat swab specimens (a 2.5-3 hr procedure), and culture. The MoAb-direct smear method was at least 86{\%}-89{\%} sensitive and 95{\%}-100{\%} specific compared with either of the other procedures. Additional prospective evaluations, as well as retrospective studies on a selected bank of slides stored from the preceding year, established that this MoAb could also be used with confidence in testing where direct smears are not employed.",
author = "Minnich, {Linda L.} and Shehab, {Ziad M} and Ray, {C. George}",
year = "1987",
doi = "10.1016/0732-8893(87)90031-9",
language = "English (US)",
volume = "7",
pages = "137--141",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Application of pooled monoclonal antibodies for 1-hr detection of respiratory syncytial virus antigen in clinical specimens

AU - Minnich, Linda L.

AU - Shehab, Ziad M

AU - Ray, C. George

PY - 1987

Y1 - 1987

N2 - A fluorescein isothiocyanate-conjugated pool of monoclonal antibodies (MoAb) to respiratory syncytial virus (RSV) was prospectively evaluated for its utility as a direct, 1-hr test for the diagnosis of RSV infection. Direct nasopharyngeal swab smears collected from 109 infants and children with acute respiratory illnesses were studied and compared with results obtained by indirect immunofluorescence using bovine polyclonal anti-RSV antibody on eluted cells derived from pooled nasopharyngeal and throat swab specimens (a 2.5-3 hr procedure), and culture. The MoAb-direct smear method was at least 86%-89% sensitive and 95%-100% specific compared with either of the other procedures. Additional prospective evaluations, as well as retrospective studies on a selected bank of slides stored from the preceding year, established that this MoAb could also be used with confidence in testing where direct smears are not employed.

AB - A fluorescein isothiocyanate-conjugated pool of monoclonal antibodies (MoAb) to respiratory syncytial virus (RSV) was prospectively evaluated for its utility as a direct, 1-hr test for the diagnosis of RSV infection. Direct nasopharyngeal swab smears collected from 109 infants and children with acute respiratory illnesses were studied and compared with results obtained by indirect immunofluorescence using bovine polyclonal anti-RSV antibody on eluted cells derived from pooled nasopharyngeal and throat swab specimens (a 2.5-3 hr procedure), and culture. The MoAb-direct smear method was at least 86%-89% sensitive and 95%-100% specific compared with either of the other procedures. Additional prospective evaluations, as well as retrospective studies on a selected bank of slides stored from the preceding year, established that this MoAb could also be used with confidence in testing where direct smears are not employed.

UR - http://www.scopus.com/inward/record.url?scp=0023192787&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023192787&partnerID=8YFLogxK

U2 - 10.1016/0732-8893(87)90031-9

DO - 10.1016/0732-8893(87)90031-9

M3 - Article

C2 - 3308304

AN - SCOPUS:0023192787

VL - 7

SP - 137

EP - 141

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 2

ER -