Applications of a colorimetric plate assay for soluble methane monooxygenase activity

D. W. Graham, D. G. Korich, R. P. LeBlanc, N. A. Sinclair, Robert G Arnold

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

A straightforward method is described for screening methanotrophic colonies for soluble methane monooxygenase (sMMO) activity on solid media. Such activity results in the development of a colored complex between 1- naphthol, which is formed when sMMO reacts with naphthalene, and o- dianisidine (tetrazotized). Methanotrophic colonies expressing sMMO turned deep purple when exposed successively to naphthalene and o-dianisidine. The method was evaluated within the contexts of two potential applications. The first was for the enumeration of Methylosinus trichosporium OB3b in a methane-amended, unsaturated soil column dedicated to vinyl chloride treatment. The second application was for the isolation and enumeration of sMMO-bearing methanotrophs from sanitary landfill soils. The technique was effective in both applications.

Original languageEnglish (US)
Pages (from-to)2231-2236
Number of pages6
JournalApplied and Environmental Microbiology
Volume58
Issue number7
StatePublished - 1992

Fingerprint

methane monooxygenase
Dianisidine
methane
assay
naphthalene
assays
Methylosinus trichosporium
Soil
vinyl chloride
1-naphthol
Waste Disposal Facilities
Vinyl Chloride
methanotrophs
Methane
landfills
soil
soil column
methodology
screening
chloride

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Applications of a colorimetric plate assay for soluble methane monooxygenase activity. / Graham, D. W.; Korich, D. G.; LeBlanc, R. P.; Sinclair, N. A.; Arnold, Robert G.

In: Applied and Environmental Microbiology, Vol. 58, No. 7, 1992, p. 2231-2236.

Research output: Contribution to journalArticle

Graham, DW, Korich, DG, LeBlanc, RP, Sinclair, NA & Arnold, RG 1992, 'Applications of a colorimetric plate assay for soluble methane monooxygenase activity', Applied and Environmental Microbiology, vol. 58, no. 7, pp. 2231-2236.
Graham, D. W. ; Korich, D. G. ; LeBlanc, R. P. ; Sinclair, N. A. ; Arnold, Robert G. / Applications of a colorimetric plate assay for soluble methane monooxygenase activity. In: Applied and Environmental Microbiology. 1992 ; Vol. 58, No. 7. pp. 2231-2236.
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