Arabidopsis cockayne syndrome a-like proteins 1A and 1B form a complex with CULLIN4 and damage DNA binding protein 1A and regulate the response to UV irradiation

Caiguo Zhang, Huiping Guo, Jun Zhang, Guangqin Guo, Karen S. Schumaker, Yan Guob

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

In plants, as in animals, DNA is constantly subject to chemical modification. UV-B irradiation is a major genotoxic agent and has significant effects on plant growth and development. Through forward genetic screening, we identified a UV-B- sensitive mutant (csaat1a-3) in Arabidopsis thaliana, in which expression of CSAat1A, encoding a Cockayne Syndrome A-like protein, is reduced due to insertion of a T-DNA in the promoter region. Arabidopsis lacking CSAat1A or its homolog CSAat1B is more sensitive to UV-B and the genotoxic drug methyl methanesulfonate and exhibits reduced transcriptioncoupled repair activity. Yeast two-hybrid analysis indicated that both CSAat1A and B interact with DDB1A (UV-Damage DNA Binding Protein1). Coimmunoprecipitation assays demonstrated that CSAat1A and B associate with the CULLIN4 (CUL4)- DDB1A complex in Arabidopsis. A split-yellow fluorescent protein assay showed that this interaction occurs in the nucleus, consistent with the idea that the CUL4-DDB1A-CSA complex functions as a nuclear E3 ubiquitin ligase. CSAat1A and B formed heterotetramers in Arabidopsis. Taken together, our data suggest that the plant CUL4-DDB1ACSAat1A and B complex represents a unique mechanism to promote ubiquitination of substrates in response to DNA damage.

Original languageEnglish (US)
Pages (from-to)2353-2369
Number of pages17
JournalPlant Cell
Volume22
Issue number7
DOIs
StatePublished - Jul 2010

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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