Abstract
This paper investigates the architecture dependence of endothelial cell networks on collective calcium signaling. Upon exogenous histamine stimulation, differential calcium responses are observed in human umbilical vein endothelial cells (IIUVEC) cultured monolayers, plasma lithography patterned hexagon cell networks, and capillary-like structures (CLS) self-organized on matrigel. By systematically modulating the cell-cell interaction with geometrical patterning and the gap-junction intercellular communication with pharmacological reagents, our results reveal the emergent network property of endothelial cells in response to extracellular chemical signals.
| Original language | English (US) |
|---|---|
| Title of host publication | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 428-430 |
| Number of pages | 3 |
| Volume | 1 |
| ISBN (Print) | 9781632666246 |
| State | Published - 2013 |
| Event | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: Oct 27 2013 → Oct 31 2013 |
Other
| Other | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
|---|---|
| Country | Germany |
| City | Freiburg |
| Period | 10/27/13 → 10/31/13 |
Fingerprint
Keywords
- Architecture
- Calcium signaling
- Endothelial cells
- Plasma lithography
ASJC Scopus subject areas
- Bioengineering
Cite this
Architecture-dependent collective calcium signaling in microengineered and self-organized endothelial cell networks. / Sun, J.; Wong, Pak Kin.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 428-430.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Architecture-dependent collective calcium signaling in microengineered and self-organized endothelial cell networks
AU - Sun, J.
AU - Wong, Pak Kin
PY - 2013
Y1 - 2013
N2 - This paper investigates the architecture dependence of endothelial cell networks on collective calcium signaling. Upon exogenous histamine stimulation, differential calcium responses are observed in human umbilical vein endothelial cells (IIUVEC) cultured monolayers, plasma lithography patterned hexagon cell networks, and capillary-like structures (CLS) self-organized on matrigel. By systematically modulating the cell-cell interaction with geometrical patterning and the gap-junction intercellular communication with pharmacological reagents, our results reveal the emergent network property of endothelial cells in response to extracellular chemical signals.
AB - This paper investigates the architecture dependence of endothelial cell networks on collective calcium signaling. Upon exogenous histamine stimulation, differential calcium responses are observed in human umbilical vein endothelial cells (IIUVEC) cultured monolayers, plasma lithography patterned hexagon cell networks, and capillary-like structures (CLS) self-organized on matrigel. By systematically modulating the cell-cell interaction with geometrical patterning and the gap-junction intercellular communication with pharmacological reagents, our results reveal the emergent network property of endothelial cells in response to extracellular chemical signals.
KW - Architecture
KW - Calcium signaling
KW - Endothelial cells
KW - Plasma lithography
UR - http://www.scopus.com/inward/record.url?scp=84907334146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907334146&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907334146
SN - 9781632666246
VL - 1
SP - 428
EP - 430
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
ER -