Abstract
The sensitivity limits for an in-line interferometric (IL)/fluorescent (FL) dual-channel BioCD are established as a function of spatial averaging. Forward-phase and sandwich assays at 10 ng/ml were performed on large-scale antibody microarrays (6800-spot) and detected using in-line interferometry and fluorescence channels. The interferometric channel has an extrapolated label-free limit-of-detection (LOD) of 250 pg/ml in a forward-phase assay for which the fluorescent channel is inapplicable. In the sandwich assay, the extrapolated limit-of-detection is 70 pg/ml for the interferometric channel, and for the fluorescent channel it is 30 pg/ml. Intrinsic scale-free sensitivities for the detection channels are defined assuming spatially uncorrelated fluctuations with sensitivities of S′ = 13 pg/mm for interferometric detection and 7 pg/mm for sandwich fluorescent detection. The intrinsic sensitivities become weakly scale dependent in the presence of fractal spatial correlations among the antibody spots.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 981-987 |
| Number of pages | 7 |
| Journal | Biosensors and Bioelectronics |
| Volume | 24 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 1 2008 |
| Externally published | Yes |
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Keywords
- Antibody microarray
- BioCD
- Fluorescence
- Immunoassay
- Interferometry
- Label-free
- Scalability
ASJC Scopus subject areas
- Biophysics
- Biomedical Engineering
- Biotechnology
- Electrochemistry
Cite this
Area-scaling of interferometric and fluorescent detection of protein on antibody microarrays. / Wang, Xuefeng; Zhao, Ming; Nolte, David D.
In: Biosensors and Bioelectronics, Vol. 24, No. 4, 01.12.2008, p. 981-987.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Area-scaling of interferometric and fluorescent detection of protein on antibody microarrays
AU - Wang, Xuefeng
AU - Zhao, Ming
AU - Nolte, David D.
PY - 2008/12/1
Y1 - 2008/12/1
N2 - The sensitivity limits for an in-line interferometric (IL)/fluorescent (FL) dual-channel BioCD are established as a function of spatial averaging. Forward-phase and sandwich assays at 10 ng/ml were performed on large-scale antibody microarrays (6800-spot) and detected using in-line interferometry and fluorescence channels. The interferometric channel has an extrapolated label-free limit-of-detection (LOD) of 250 pg/ml in a forward-phase assay for which the fluorescent channel is inapplicable. In the sandwich assay, the extrapolated limit-of-detection is 70 pg/ml for the interferometric channel, and for the fluorescent channel it is 30 pg/ml. Intrinsic scale-free sensitivities for the detection channels are defined assuming spatially uncorrelated fluctuations with sensitivities of S′ = 13 pg/mm for interferometric detection and 7 pg/mm for sandwich fluorescent detection. The intrinsic sensitivities become weakly scale dependent in the presence of fractal spatial correlations among the antibody spots.
AB - The sensitivity limits for an in-line interferometric (IL)/fluorescent (FL) dual-channel BioCD are established as a function of spatial averaging. Forward-phase and sandwich assays at 10 ng/ml were performed on large-scale antibody microarrays (6800-spot) and detected using in-line interferometry and fluorescence channels. The interferometric channel has an extrapolated label-free limit-of-detection (LOD) of 250 pg/ml in a forward-phase assay for which the fluorescent channel is inapplicable. In the sandwich assay, the extrapolated limit-of-detection is 70 pg/ml for the interferometric channel, and for the fluorescent channel it is 30 pg/ml. Intrinsic scale-free sensitivities for the detection channels are defined assuming spatially uncorrelated fluctuations with sensitivities of S′ = 13 pg/mm for interferometric detection and 7 pg/mm for sandwich fluorescent detection. The intrinsic sensitivities become weakly scale dependent in the presence of fractal spatial correlations among the antibody spots.
KW - Antibody microarray
KW - BioCD
KW - Fluorescence
KW - Immunoassay
KW - Interferometry
KW - Label-free
KW - Scalability
UR - http://www.scopus.com/inward/record.url?scp=57349156445&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57349156445&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2008.07.078
DO - 10.1016/j.bios.2008.07.078
M3 - Article
C2 - 18809308
AN - SCOPUS:57349156445
VL - 24
SP - 981
EP - 987
JO - Biosensors
JF - Biosensors
SN - 0956-5663
IS - 4
ER -