Arf6 and Rab22 mediate T cell conjugate formation by regulating clathrin-independent endosomal membrane trafficking

Debra L. Johnson, Jessica Wayt, Jean M. Wilson, Julie G. Donaldson

Research output: Research - peer-reviewArticle

Abstract

Endosomal trafficking can influence the composition of the plasma membrane and the ability of cells to polarize their membranes. Here, we examined whether trafficking through clathrin-independent endocytosis (CIE) affects the ability of T cells to form a cell-cell conjugate with antigen-presenting cells (APCs). We show that CIE occurs in both the Jurkat T cell line and primary human T cells. In Jurkat cells, the activities of two guanine nucleotide binding proteins, Arf6 and Rab22 (also known as Rab22a), influence CIE and conjugate formation. Expression of the constitutively active form of Arf6, Arf6Q67L, inhibits CIE and conjugate formation, and results in the accumulation of vacuoles containing lymphocyte functionassociated antigen 1 (LFA-1) and CD4, molecules important for T cell interaction with the APC. Moreover, expression of the GTPbinding defective mutant of Rab22, Rab22S19N, inhibits CIE and conjugate formation, suggesting that Rab22 function is required for these activities. Furthermore, Jurkat cells expressing Rab22S19N were impaired in spreading onto coverslips coated with T cell receptor-activating antibodies. These observations support a role for CIE, Arf6 and Rab22 in conjugate formation between T cells and APCs.

LanguageEnglish (US)
Pages2405-2415
Number of pages11
JournalJournal of Cell Science
Volume130
Issue number14
DOIs
StatePublished - Jul 15 2017

Fingerprint

Clathrin
Endocytosis
T-Lymphocytes
Membranes
Jurkat Cells
Antigen-Presenting Cells
CD4 Antigens
Guanine Nucleotides
Viral Tumor Antigens
T-Cell Antigen Receptor
Vacuoles
Cell Communication
Carrier Proteins
Cell Membrane
Lymphocytes
Antigens
Cell Line
Antibodies

Keywords

  • Arf6
  • Clathrin-independent endocytosis
  • Immunological synapse
  • Rab22
  • Rab22a
  • T cell

ASJC Scopus subject areas

  • Cell Biology

Cite this

Arf6 and Rab22 mediate T cell conjugate formation by regulating clathrin-independent endosomal membrane trafficking. / Johnson, Debra L.; Wayt, Jessica; Wilson, Jean M.; Donaldson, Julie G.

In: Journal of Cell Science, Vol. 130, No. 14, 15.07.2017, p. 2405-2415.

Research output: Research - peer-reviewArticle

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