Arsenic upregulates MMP-9 and inhibits wound repair in human airway epithelial cells

Colin E. Olsen, Andrew E. Liguori, Yue Zong, R. Clark Lantz, Jefferey L. Burgess, Scott Boitano

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

As part of the innate immune defense, the polarized conducting lung epithelium acts as a barrier to keep particulates carried in respiration from underlying tissue. Arsenic is a metalloid toxicant that can affect the lung via inhalation or ingestion. We have recently shown that chronic exposure of mice or humans to arsenic (10-50 ppb) in drinking water alters bronchiolar lavage or sputum proteins consistent with reduced epithelial cell migration and wound repair in the airway. In this report, we used an in vitro model to examine effects of acute exposure of arsenic (15-290 ppb) on conducting airway lung epithelium. We found that arsenic at concentrations as low as 30 ppb inhibits reformation of the epithelial monolayer following scrape wounds of monolayer cultures. In an effort to understand functional contributions to epithelial wound repair altered by arsenic, we showed that acute arsenic exposure increases activity and expression of matrix metalloproteinase (MMP)-9, an important protease in lung function. Furthermore, inhibition of MMP-9 in arsenic-treated cells improved wound repair. We propose that arsenic in the airway can alter the airway epithelial barrier by restricting proper wound repair in part through the upregulation of MMP-9 by lung epithelial cells.

Original languageEnglish (US)
Pages (from-to)L293-L302
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume295
Issue number2
DOIs
StatePublished - Aug 2008

Keywords

  • 16HBE14o-cells
  • Airway epithelial barrier
  • Cell migration
  • Matrix metalloproteinase
  • Sodium arsenite

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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