Assessment of constitutive activity in E-Type prostanoid receptors

Hiromichi Fujino, Toshihiko Murayama, John W Regan

Research output: Book/ReportBook

1 Citation (Scopus)

Abstract

The potential for G-protein-coupled receptors (GPCRs) to show constitutive activity is emerging as one of the fundamental properties of GPCRs signal transduction. Indeed, of the four subtypes of E-type prostanoid (EP) receptors, the EP3 and EP4 subtypes show constitutive activity in addition to their innate ligand-dependent activation of signaling pathways. The constitutive activity of the EP3 and EP4 receptor subtypes was discovered during the initial characterizations of these receptors and may be important for setting the basal level of cellular tone in the given signaling pathway. This chapter introduces some of the methods that can be used to study the constitutive activity of the EP receptors.

Original languageEnglish (US)
PublisherUnknown Publisher
Number of pages13
Volume484
EditionC
DOIs
StatePublished - 2010

Publication series

NameMethods in Enzymology
No.C
Volume484
ISSN (Print)00766879

Fingerprint

G-Protein-Coupled Receptors
Prostaglandins
Receptors, Prostaglandin E, EP3 Subtype
Receptors, Prostaglandin E, EP4 Subtype
Signal transduction
Signal Transduction
Chemical activation
Ligands

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Fujino, H., Murayama, T., & Regan, J. W. (2010). Assessment of constitutive activity in E-Type prostanoid receptors. (C ed.) (Methods in Enzymology; Vol. 484, No. C). Unknown Publisher. https://doi.org/10.1016/B978-0-12-381298-8.00005-8

Assessment of constitutive activity in E-Type prostanoid receptors. / Fujino, Hiromichi; Murayama, Toshihiko; Regan, John W.

C ed. Unknown Publisher, 2010. 13 p. (Methods in Enzymology; Vol. 484, No. C).

Research output: Book/ReportBook

Fujino, H, Murayama, T & Regan, JW 2010, Assessment of constitutive activity in E-Type prostanoid receptors. Methods in Enzymology, no. C, vol. 484, vol. 484, C edn, Unknown Publisher. https://doi.org/10.1016/B978-0-12-381298-8.00005-8
Fujino H, Murayama T, Regan JW. Assessment of constitutive activity in E-Type prostanoid receptors. C ed. Unknown Publisher, 2010. 13 p. (Methods in Enzymology; C). https://doi.org/10.1016/B978-0-12-381298-8.00005-8
Fujino, Hiromichi ; Murayama, Toshihiko ; Regan, John W. / Assessment of constitutive activity in E-Type prostanoid receptors. C ed. Unknown Publisher, 2010. 13 p. (Methods in Enzymology; C).
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