Assignment of the gene for the β subunit of thyroid-stimulating hormone to the short arm of human chromosome 1

N. C. Dracopoli, W. J. Rettig, G Kerr Whitfield, G. J. Darlington, B. A. Spengler, J. L. Biedler, L. J. Old, I. A. Kourides

Research output: Contribution to journalArticle

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Abstract

The chromosomal locations of the genes for the β subunit of human thyroid-stimulating hormone (TSH) and the glycoprotein hormone α subunit have been determined by restriction enzyme analysis of DNA extracted from rodent-human somatic cell hybrids. Human chorionic gonadotropin (CG) α-subunit cDNA and a cloned 0.9-kilobase (kb) fragment of the human TSH β-subunit gene were used as hybridization probes in the analysis of Southern blots of DNA extracted from rodent-human hybrid clones. Analysis of the segregation of 5- and 10-kb EcoRI fragments hybridizing to CG α-subunit cDNA confirmed the previous assignment of this gene to chromosome 6. Analysis of the patterns of segregation of a 2.3-kb EcoRI fragment containing human TSH β-subunit sequences permitted the assignment of the TSH β-subunit gene to human chromosome 1. The subregional assignment of TSH β subunit to chromosome 1p22 was made possible by the additional analysis of a set of hybrids containing partially overlapping segments of this chromosome. Human TSH β subunit is not syntenic with genes encoding the β subunits of CG, luteinizing hormone, or follicle-stimulating hormone and is assigned to a conserved linkage group that also contains the structural genes for the β subunit of nerve growth factor (NGFB) and the proto-oncogene N-ras (NRAS).

Original languageEnglish (US)
Pages (from-to)1822-1826
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number6
StatePublished - 1986
Externally publishedYes

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Chromosomes, Human, Pair 1
Human Chromosomes
Thyrotropin
Chorionic Gonadotropin
Genes
Rodentia
Complementary DNA
Chromosomes
Restriction Mapping
Chromosomes, Human, Pair 6
Proto-Oncogenes
Hybrid Cells
DNA
Follicle Stimulating Hormone
Nerve Growth Factor
Luteinizing Hormone
Southern Blotting
Glycoproteins
Clone Cells
Hormones

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Assignment of the gene for the β subunit of thyroid-stimulating hormone to the short arm of human chromosome 1. / Dracopoli, N. C.; Rettig, W. J.; Whitfield, G Kerr; Darlington, G. J.; Spengler, B. A.; Biedler, J. L.; Old, L. J.; Kourides, I. A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 6, 1986, p. 1822-1826.

Research output: Contribution to journalArticle

Dracopoli, N. C. ; Rettig, W. J. ; Whitfield, G Kerr ; Darlington, G. J. ; Spengler, B. A. ; Biedler, J. L. ; Old, L. J. ; Kourides, I. A. / Assignment of the gene for the β subunit of thyroid-stimulating hormone to the short arm of human chromosome 1. In: Proceedings of the National Academy of Sciences of the United States of America. 1986 ; Vol. 83, No. 6. pp. 1822-1826.
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abstract = "The chromosomal locations of the genes for the β subunit of human thyroid-stimulating hormone (TSH) and the glycoprotein hormone α subunit have been determined by restriction enzyme analysis of DNA extracted from rodent-human somatic cell hybrids. Human chorionic gonadotropin (CG) α-subunit cDNA and a cloned 0.9-kilobase (kb) fragment of the human TSH β-subunit gene were used as hybridization probes in the analysis of Southern blots of DNA extracted from rodent-human hybrid clones. Analysis of the segregation of 5- and 10-kb EcoRI fragments hybridizing to CG α-subunit cDNA confirmed the previous assignment of this gene to chromosome 6. Analysis of the patterns of segregation of a 2.3-kb EcoRI fragment containing human TSH β-subunit sequences permitted the assignment of the TSH β-subunit gene to human chromosome 1. The subregional assignment of TSH β subunit to chromosome 1p22 was made possible by the additional analysis of a set of hybrids containing partially overlapping segments of this chromosome. Human TSH β subunit is not syntenic with genes encoding the β subunits of CG, luteinizing hormone, or follicle-stimulating hormone and is assigned to a conserved linkage group that also contains the structural genes for the β subunit of nerve growth factor (NGFB) and the proto-oncogene N-ras (NRAS).",
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AU - Dracopoli, N. C.

AU - Rettig, W. J.

AU - Whitfield, G Kerr

AU - Darlington, G. J.

AU - Spengler, B. A.

AU - Biedler, J. L.

AU - Old, L. J.

AU - Kourides, I. A.

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N2 - The chromosomal locations of the genes for the β subunit of human thyroid-stimulating hormone (TSH) and the glycoprotein hormone α subunit have been determined by restriction enzyme analysis of DNA extracted from rodent-human somatic cell hybrids. Human chorionic gonadotropin (CG) α-subunit cDNA and a cloned 0.9-kilobase (kb) fragment of the human TSH β-subunit gene were used as hybridization probes in the analysis of Southern blots of DNA extracted from rodent-human hybrid clones. Analysis of the segregation of 5- and 10-kb EcoRI fragments hybridizing to CG α-subunit cDNA confirmed the previous assignment of this gene to chromosome 6. Analysis of the patterns of segregation of a 2.3-kb EcoRI fragment containing human TSH β-subunit sequences permitted the assignment of the TSH β-subunit gene to human chromosome 1. The subregional assignment of TSH β subunit to chromosome 1p22 was made possible by the additional analysis of a set of hybrids containing partially overlapping segments of this chromosome. Human TSH β subunit is not syntenic with genes encoding the β subunits of CG, luteinizing hormone, or follicle-stimulating hormone and is assigned to a conserved linkage group that also contains the structural genes for the β subunit of nerve growth factor (NGFB) and the proto-oncogene N-ras (NRAS).

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