Association between border cell responses and localized root infection by pathogenic Aphanomyces euteiches

Marc Antoine Cannesan, Christophe Gangneux, Arnaud Lanoue, David Giron, Karine Laval, Martha C Hawes, Azeddine Driouich, Maïté Vicré-Gibouin

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

•Background and Aims: The oomycete Aphanomyces euteiches causes up to 80% crop loss in pea (Pisum sativum). Aphanomyces euteiches invades the root system leading to a complete arrest of root growth and ultimately to plant death. To date, disease control measures are limited to crop rotation and no resistant pea lines are available. The present study aims to get a deeper understanding of the early oomyceteplant interaction at the tissue and cellular levels. •Methods: Here, the process of root infection by A. euteiches on pea is investigated using flow cytometry and microscopic techniques. Dynamic changes in secondary metabolism are analysed with high-performance liquid chromatography with diode-array detection. •Key Results: Root infection is initiated in the elongation zone but not in the root cap and border cells. Border-cell production is significantly enhanced in response to root inoculation with changes in their size and morphology. The stimulatory effect of A. euteiches on border-cell production is dependent on the number of oospores inoculated. Interestingly, border cells respond to pathogen challenge by increasing the synthesis of the phytoalexin pisatin. •Conclusions: Distinctive responses to A. euteiches inoculation occur at the root tissue level. The findings suggest that root border cells in pea are involved in local defence of the root tip against A. euteiches. Root border cells constitute a convenient quantitative model to measure the molecular and cellular basis of plantmicrobe interactions.

Original languageEnglish (US)
Pages (from-to)459-469
Number of pages11
JournalAnnals of Botany
Volume108
Issue number3
DOIs
StatePublished - Sep 2011

Fingerprint

Aphanomyces euteiches
peas
infection
cells
pisatin
root inoculation
oospores
root cap
crop losses
Oomycetes
phytoalexins
root tips
Pisum sativum
root systems
flow cytometry
control methods
root growth
disease control
high performance liquid chromatography
death

Keywords

  • Aphanomyces euteiches
  • pathogen
  • phenolics
  • pisatin
  • Pisum sativum
  • root border cells
  • root cap
  • root infection

ASJC Scopus subject areas

  • Plant Science

Cite this

Cannesan, M. A., Gangneux, C., Lanoue, A., Giron, D., Laval, K., Hawes, M. C., ... Vicré-Gibouin, M. (2011). Association between border cell responses and localized root infection by pathogenic Aphanomyces euteiches. Annals of Botany, 108(3), 459-469. https://doi.org/10.1093/aob/mcr177

Association between border cell responses and localized root infection by pathogenic Aphanomyces euteiches. / Cannesan, Marc Antoine; Gangneux, Christophe; Lanoue, Arnaud; Giron, David; Laval, Karine; Hawes, Martha C; Driouich, Azeddine; Vicré-Gibouin, Maïté.

In: Annals of Botany, Vol. 108, No. 3, 09.2011, p. 459-469.

Research output: Contribution to journalArticle

Cannesan, MA, Gangneux, C, Lanoue, A, Giron, D, Laval, K, Hawes, MC, Driouich, A & Vicré-Gibouin, M 2011, 'Association between border cell responses and localized root infection by pathogenic Aphanomyces euteiches', Annals of Botany, vol. 108, no. 3, pp. 459-469. https://doi.org/10.1093/aob/mcr177
Cannesan, Marc Antoine ; Gangneux, Christophe ; Lanoue, Arnaud ; Giron, David ; Laval, Karine ; Hawes, Martha C ; Driouich, Azeddine ; Vicré-Gibouin, Maïté. / Association between border cell responses and localized root infection by pathogenic Aphanomyces euteiches. In: Annals of Botany. 2011 ; Vol. 108, No. 3. pp. 459-469.
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