Association between cyclooxygenase expression and colorectal adenoma characteristics

Janine G Einspahr, Robert S Krouse, Ji Min Yochim, Peter V. Danenberg, Kathleen D. Danenberg, Achyut K Bhattacharyya, María E. Martínez, David S Alberts

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Abstract

The cyclooxygenase (COX) pathway is important in colorectal carcinogenesis with the majority of cancers overexpressing COX-2; however, the role of COX-2 in the development of colorectal adenomas is less well defined. Accordingly, we analyzed 108 colorectal adenomas for COX-1 and COX-2 transcription in archival formalin-fixed, paraffin-embedded tissue using by real-time PCR and normalized to β-actin. Neither COX-1 nor COX-2 mRNA expression differed with regard to age or gender of the subject. COX-2 mRNA expression was significantly higher in distal adenomas (2.2 ± 1.9) compared with proximal (0.7 ± 0.5) adenomas (P < 0.0001) and in larger (≥7 mm) compared with smaller (<7 mm) adenomas (2.3 ± 2.2 and 1.7 ± 1.3, respectively, P = 0.04). COX-2 expression did not differ significantly in tubular compared with tubulovillous adenomas, although there appeared to be a trend toward higher COX-2 expression in tubulovillous adenomas with increasing villous content. Additionally, there was not a significant difference in either COX-1 or COX-2 based on the degree of dysplasia Therefore, if COX-2 inhibitors work through a COX-2 mechanism, these agents may have differential effects on colorectal adenomas that are distal and larger.

Original languageEnglish (US)
Pages (from-to)3891-3893
Number of pages3
JournalCancer Research
Volume63
Issue number14
Publication statusPublished - Jul 15 2003

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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