Association between YAP expression in neoplastic and non-neoplastic breast tissue with arsenic urinary levels

Gladis Michel-Ramirez, Rogelio Recio-Vega, Guadalupe Ocampo-Gomez, Eduardo Palacios-Sanchez, Manuel Delgado-Macias, Manuel Delgado-Gaona, Robert Clark Lantz, Jay Gandolfi, Tania Gonzalez-Cortes

Research output: Research - peer-reviewArticle

Abstract

The Hippo pathway regulates cell proliferation and apoptosis and it has been noted that loss of critical components of this pathway can lead to uncontrolled cell growth. Yes-associated protein (YAP) is an important component of this Hippo pathway because YAP is the nuclear effector of the Hippo tumor suppressor pathway and it is crucial for the response to oxidative stress induced by cellular process and by different xenobiotics, including arsenic. It has been proposed that YAP dysregulation can contribute to a malignant cellular phenotype acting as both a tumor suppressor and an oncogene. The aim of the study was to assess and compare the expression of YAP in neoplastic and non-neoplastic breast tissue of women chronically exposed to arsenic through drinking water. YAP expression was assessed by immunohistochemistry in 120 breast biopsies from women with breast cancer and from women with other non-neoplastic breast pathologies. Arsenic concentration was quantified in urine. The results disclosed a significant lower percentage of cytoplasm YAP expression in cases and that YAP high-intensity staining in the cytoplasm but not in the nucleus decreases the risk for breast cancer. In conclusion, our overall data suggest that YAP may act as a tumor suppressor protein because their reduced expression in cases, which can induce an environment favorable for inhibition of apoptosis and promoting cellular proliferation by increasing genetic instability of cells, which might contribute to the pathogenesis of cancer.

LanguageEnglish (US)
JournalJournal of Applied Toxicology
DOIs
StateAccepted/In press - 2017

Fingerprint

Arsenic
Breast
Proteins
Tissue
Neoplasms
Cytoplasm
Cell Proliferation
Apoptosis
Breast Neoplasms
Tumors
Tumor Suppressor Proteins
Critical Pathways
Xenobiotics
Nuclear Proteins
Oncogenes
Drinking Water
Oxidative Stress
Immunohistochemistry
Urine
Pathology

Keywords

  • Arsenic
  • Breast cancer
  • YAP
  • Yes Associated Protein

ASJC Scopus subject areas

  • Toxicology

Cite this

Michel-Ramirez, G., Recio-Vega, R., Ocampo-Gomez, G., Palacios-Sanchez, E., Delgado-Macias, M., Delgado-Gaona, M., ... Gonzalez-Cortes, T. (2017). Association between YAP expression in neoplastic and non-neoplastic breast tissue with arsenic urinary levels. Journal of Applied Toxicology. DOI: 10.1002/jat.3481

Association between YAP expression in neoplastic and non-neoplastic breast tissue with arsenic urinary levels. / Michel-Ramirez, Gladis; Recio-Vega, Rogelio; Ocampo-Gomez, Guadalupe; Palacios-Sanchez, Eduardo; Delgado-Macias, Manuel; Delgado-Gaona, Manuel; Lantz, Robert Clark; Gandolfi, Jay; Gonzalez-Cortes, Tania.

In: Journal of Applied Toxicology, 2017.

Research output: Research - peer-reviewArticle

Michel-Ramirez, G, Recio-Vega, R, Ocampo-Gomez, G, Palacios-Sanchez, E, Delgado-Macias, M, Delgado-Gaona, M, Lantz, RC, Gandolfi, J & Gonzalez-Cortes, T 2017, 'Association between YAP expression in neoplastic and non-neoplastic breast tissue with arsenic urinary levels' Journal of Applied Toxicology. DOI: 10.1002/jat.3481
Michel-Ramirez G, Recio-Vega R, Ocampo-Gomez G, Palacios-Sanchez E, Delgado-Macias M, Delgado-Gaona M et al. Association between YAP expression in neoplastic and non-neoplastic breast tissue with arsenic urinary levels. Journal of Applied Toxicology. 2017. Available from, DOI: 10.1002/jat.3481
Michel-Ramirez, Gladis ; Recio-Vega, Rogelio ; Ocampo-Gomez, Guadalupe ; Palacios-Sanchez, Eduardo ; Delgado-Macias, Manuel ; Delgado-Gaona, Manuel ; Lantz, Robert Clark ; Gandolfi, Jay ; Gonzalez-Cortes, Tania. / Association between YAP expression in neoplastic and non-neoplastic breast tissue with arsenic urinary levels. In: Journal of Applied Toxicology. 2017
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