Association of ERp57 with mouse MHC class I molecules is tapasin dependent and mimics that of calreticulin and not calnexin

Michael R. Harris, Lonnie Lybarger, Yik Y L Yu, Nancy B. Myers, Ted H. Hansen

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Before peptide binding in the endoplasmic reticulum, the class I heavy (H) chain-β2-microglobulin complexes are detected in association with TAP and two chaperones, TPN and CRT. Recent studies have shown that the thiol-dependent reductase, ERp57, is also present in this peptide-loading complex. However, it remains controversial whether the association of ERp57 with MHC class I molecules precedes their combined association with the peptide-loading complex or whether ERp57 only associates with class I molecules in the presence of TPN. Resolution of this controversy could help determine the role of ERp57 in class I folding and/or assembly. To define the mouse class I H chain structures involved in interaction with ERp57, we tested chaperone association of Ld mutations at residues 134 and 227/229 (previously implicated in TAP association), residues 86/88 (which ablate an N-linked glycan), and residue 101 (which disrupts a disulfide bond). The association of ERp57 with each of these mutant H chains showed a complete concordance with CRT, TAP, and TPN but not with calnexin. Furthermore, ERp57 failed to associate with H chain in TPN-deficient .220 cells. These combined data demonstrate that, during the assembly of the peptide-loading complex, the association of ERp57 with mouse class I is TPN dependent and parallels that of CRT and not calnexin.

Original languageEnglish (US)
Pages (from-to)6686-6692
Number of pages7
JournalJournal of Immunology
Volume166
Issue number11
StatePublished - May 1 2001
Externally publishedYes

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Calnexin
Calreticulin
Peptides
Sulfhydryl Compounds
Endoplasmic Reticulum
Disulfides
Polysaccharides
Oxidoreductases
Mutation
tapasin

ASJC Scopus subject areas

  • Immunology

Cite this

Association of ERp57 with mouse MHC class I molecules is tapasin dependent and mimics that of calreticulin and not calnexin. / Harris, Michael R.; Lybarger, Lonnie; Yu, Yik Y L; Myers, Nancy B.; Hansen, Ted H.

In: Journal of Immunology, Vol. 166, No. 11, 01.05.2001, p. 6686-6692.

Research output: Contribution to journalArticle

Harris, Michael R. ; Lybarger, Lonnie ; Yu, Yik Y L ; Myers, Nancy B. ; Hansen, Ted H. / Association of ERp57 with mouse MHC class I molecules is tapasin dependent and mimics that of calreticulin and not calnexin. In: Journal of Immunology. 2001 ; Vol. 166, No. 11. pp. 6686-6692.
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