Association of origin binding protein and single strand DNA-binding protein, ICP8, during herpes simplex virus type 1 DNA replication in vivo

Paul E Boehmer, Marianne C. Craigie, Nigel D. Stow, I. R. Lehman

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The herpes simplex virus type 1 (HSV-1) origin binding protein (UL9 protein) interacts specifically with the HSV-1 encoded single strand DNA- binding protein ICP8 (Boehmer, P. E. and Lehman, I. R. (1994) Proc. Natl. Acad. Sci. U.S.A. 90. 8444-8448). A UL9 mutant protein (UL9DM27) that lacks the C-terminal 27 amino acids shows normal origin-specific DNA binding and retains its DNA-dependent ATPase and helicase activities, but has a greatly reduced affinity for ICP8. The extreme C-terminal portion of the UL9 protein is therefore required for ICP8 binding. The helicase activity of the UL9DM27 protein is approximately 8-fold greater than that of the wild type UL9 protein and is not stimulated by ICP8. The UL9DM27 protein has a reduced ability to replicate origin-containing plasmids in vivo. Consequently, the interaction between the UL9 protein and ICP8 is likely to be important for origin-dependent DNA replication in vivo, presumably to promote efficient unwinding of the DNA at an HSV-1 origin of DNA replication.

Original languageEnglish (US)
Pages (from-to)29329-29334
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number46
StatePublished - Nov 18 1994
Externally publishedYes

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DNA-Binding Proteins
Human Herpesvirus 1
DNA Replication
Viruses
Carrier Proteins
DNA
Proteins
DNA Helicases
Replication Origin
Mutant Proteins
Adenosine Triphosphatases
Plasmids
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Association of origin binding protein and single strand DNA-binding protein, ICP8, during herpes simplex virus type 1 DNA replication in vivo. / Boehmer, Paul E; Craigie, Marianne C.; Stow, Nigel D.; Lehman, I. R.

In: Journal of Biological Chemistry, Vol. 269, No. 46, 18.11.1994, p. 29329-29334.

Research output: Contribution to journalArticle

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AU - Lehman, I. R.

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