Asymmetric drug binding in an ATP-loaded inward-facing state of an ABC transporter

Tarjani M. Thaker, Smriti Mishra, Wenchang Zhou, Michael Mohan, Qingyu Tang, José D. Faraldo-Goméz, Hassane S. Mchaourab, Thomas M. Tomasiak

Research output: Contribution to journalArticlepeer-review

Abstract

Substrate efflux by ATP-binding cassette (ABC) transporters, which play a major role in multidrug resistance, entails the ATP-powered interconversion between transporter intermediates. Despite recent progress in structure elucidation, a number of intermediates have yet to be visualized and mechanistically interpreted. Here, we combine cryogenic-electron microscopy (cryo-EM), double electron–electron resonance spectroscopy and molecular dynamics simulations to profile a previously unobserved intermediate of BmrCD, a heterodimeric multidrug ABC exporter from Bacillus subtilis. In our cryo-EM structure, ATP-bound BmrCD adopts an inward-facing architecture featuring two molecules of the substrate Hoechst-33342 in a striking asymmetric head-to-tail arrangement. Deletion of the extracellular domain capping the substrate-binding chamber or mutation of Hoechst-coordinating residues abrogates cooperative stimulation of ATP hydrolysis. Together, our findings support a mechanistic role for symmetry mismatch between the nucleotide binding and the transmembrane domains in the conformational cycle of ABC transporters and is of notable importance for rational design of molecules for targeted ABC transporter inhibition. [Figure not available: see fulltext.].

Original languageEnglish (US)
Pages (from-to)226-235
Number of pages10
JournalNature chemical biology
Volume18
Issue number2
DOIs
StatePublished - Feb 2022

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Asymmetric drug binding in an ATP-loaded inward-facing state of an ABC transporter'. Together they form a unique fingerprint.

Cite this