Asymmetric properties of the Chlamydomonas reinhardtii cytoskeleton direct rhodopsin photoreceptor localization

Telsa M. Mittelmeier, Joseph S. Boyd, Mary Rose Lamb, Carol L Dieckmann

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The eyespot of the unicellular green alga Chlamydomonas reinhardtii is a photoreceptive organelle required for phototaxis. Relative to the anterior flagella, the eyespot is asymmetrically positioned adjacent to the daughter four-membered rootlet (D4), a unique bundle of acetylated microtubules extending from the daughter basal body toward the posterior of the cell. Here, we detail the relationship between the rhodopsin eyespot photoreceptor Channelrhodopsin 1 (ChR1) and acetylated microtubules. In wild-type cells, ChR1 was observed in an equatorial patch adjacent to D4 near the end of the acetylated microtubules and along the D4 rootlet. In cells with cytoskeletal protein mutations, supernumerary ChR1 patches remained adjacent to acetylated microtubules. In mlt1 (multieyed) mutant cells, supernumerary photoreceptor patches were not restricted to the D4 rootlet, and more anterior eyespots correlated with shorter acetylated microtubule rootlets. The data suggest a model in which photoreceptor localization is dependent on microtubule-based trafficking selective for the D4 rootlet, which is perturbed in mlt1 mutant cells.

Original languageEnglish (US)
Pages (from-to)741-753
Number of pages13
JournalJournal of Cell Biology
Volume193
Issue number4
DOIs
StatePublished - May 16 2011

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Chlamydomonas reinhardtii
Rhodopsin
Cytoskeleton
Microtubules
Basal Bodies
Photoreceptor Cells
Chlorophyta
Cytoskeletal Proteins
Flagella
Organelles
Mutation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Asymmetric properties of the Chlamydomonas reinhardtii cytoskeleton direct rhodopsin photoreceptor localization. / Mittelmeier, Telsa M.; Boyd, Joseph S.; Lamb, Mary Rose; Dieckmann, Carol L.

In: Journal of Cell Biology, Vol. 193, No. 4, 16.05.2011, p. 741-753.

Research output: Contribution to journalArticle

Mittelmeier, Telsa M. ; Boyd, Joseph S. ; Lamb, Mary Rose ; Dieckmann, Carol L. / Asymmetric properties of the Chlamydomonas reinhardtii cytoskeleton direct rhodopsin photoreceptor localization. In: Journal of Cell Biology. 2011 ; Vol. 193, No. 4. pp. 741-753.
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