Attempts to establish experimental Cyclospora cayetanensis infection in laboratory animals

Mark L. Eberhard, Ynes R. Ortega, Darcy E. Hanes, Eva K. Nace, Rose Quy Do, Martin G. Robl, Kimberly Y. Won, Cesar Gavidia, Neil L. Sass, Keith Mansfield, Alfonso Gozalo, Jeffrey Griffiths, Robert Gilman, Charles R Sterling, Michael J. Arrowood

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Attempts were made to develop an animal model for Cyclospora cayetanensis to identify a practical laboratory host for studying human cyclosporiasis. Oocysts collected from stool of infected humans in the United States, Haiti, Guatemala, Peru, and Nepal were held in potassium dichromate solution to allow development of sporozoites. The following animal types were inoculated: 9 strains of mice, including adult and neonatal immunocompetent and immune-deficient inbred and outbred strains, rats, sandrats, chickens, ducks, rabbits, jirds, hamsters, ferrets, pigs, dogs, owl monkeys, rhesus monkeys, and cynomolgus monkeys. Most animals were inoculated by gavage, although some of the primates were fed oocysts on food items. The animals were examined for signs of infection, particularly diarrhea, and stool samples were examined for 4-6 wk after inoculation. None of the animals developed patent infections or signs of infection. We conclude that none of the animals tested is susceptible to infection with C. cayetanensis.

Original languageEnglish (US)
Pages (from-to)577-582
Number of pages6
JournalJournal of Parasitology
Volume86
Issue number3
StatePublished - Jun 2000

Fingerprint

Cyclospora cayetanensis
Cyclospora
Laboratory Animals
laboratory animals
animal
Infection
infection
Oocysts
animals
oocysts
Cyclosporiasis
Aotidae
Potassium Dichromate
Inbred Strains Rats
Haiti
Guatemala
Aotus (Cebidae)
Meriones
Sporozoites
Nepal

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Eberhard, M. L., Ortega, Y. R., Hanes, D. E., Nace, E. K., Do, R. Q., Robl, M. G., ... Arrowood, M. J. (2000). Attempts to establish experimental Cyclospora cayetanensis infection in laboratory animals. Journal of Parasitology, 86(3), 577-582.

Attempts to establish experimental Cyclospora cayetanensis infection in laboratory animals. / Eberhard, Mark L.; Ortega, Ynes R.; Hanes, Darcy E.; Nace, Eva K.; Do, Rose Quy; Robl, Martin G.; Won, Kimberly Y.; Gavidia, Cesar; Sass, Neil L.; Mansfield, Keith; Gozalo, Alfonso; Griffiths, Jeffrey; Gilman, Robert; Sterling, Charles R; Arrowood, Michael J.

In: Journal of Parasitology, Vol. 86, No. 3, 06.2000, p. 577-582.

Research output: Contribution to journalArticle

Eberhard, ML, Ortega, YR, Hanes, DE, Nace, EK, Do, RQ, Robl, MG, Won, KY, Gavidia, C, Sass, NL, Mansfield, K, Gozalo, A, Griffiths, J, Gilman, R, Sterling, CR & Arrowood, MJ 2000, 'Attempts to establish experimental Cyclospora cayetanensis infection in laboratory animals', Journal of Parasitology, vol. 86, no. 3, pp. 577-582.
Eberhard ML, Ortega YR, Hanes DE, Nace EK, Do RQ, Robl MG et al. Attempts to establish experimental Cyclospora cayetanensis infection in laboratory animals. Journal of Parasitology. 2000 Jun;86(3):577-582.
Eberhard, Mark L. ; Ortega, Ynes R. ; Hanes, Darcy E. ; Nace, Eva K. ; Do, Rose Quy ; Robl, Martin G. ; Won, Kimberly Y. ; Gavidia, Cesar ; Sass, Neil L. ; Mansfield, Keith ; Gozalo, Alfonso ; Griffiths, Jeffrey ; Gilman, Robert ; Sterling, Charles R ; Arrowood, Michael J. / Attempts to establish experimental Cyclospora cayetanensis infection in laboratory animals. In: Journal of Parasitology. 2000 ; Vol. 86, No. 3. pp. 577-582.
@article{58f0bb83ed2941ddb73ca24a466363bc,
title = "Attempts to establish experimental Cyclospora cayetanensis infection in laboratory animals",
abstract = "Attempts were made to develop an animal model for Cyclospora cayetanensis to identify a practical laboratory host for studying human cyclosporiasis. Oocysts collected from stool of infected humans in the United States, Haiti, Guatemala, Peru, and Nepal were held in potassium dichromate solution to allow development of sporozoites. The following animal types were inoculated: 9 strains of mice, including adult and neonatal immunocompetent and immune-deficient inbred and outbred strains, rats, sandrats, chickens, ducks, rabbits, jirds, hamsters, ferrets, pigs, dogs, owl monkeys, rhesus monkeys, and cynomolgus monkeys. Most animals were inoculated by gavage, although some of the primates were fed oocysts on food items. The animals were examined for signs of infection, particularly diarrhea, and stool samples were examined for 4-6 wk after inoculation. None of the animals developed patent infections or signs of infection. We conclude that none of the animals tested is susceptible to infection with C. cayetanensis.",
author = "Eberhard, {Mark L.} and Ortega, {Ynes R.} and Hanes, {Darcy E.} and Nace, {Eva K.} and Do, {Rose Quy} and Robl, {Martin G.} and Won, {Kimberly Y.} and Cesar Gavidia and Sass, {Neil L.} and Keith Mansfield and Alfonso Gozalo and Jeffrey Griffiths and Robert Gilman and Sterling, {Charles R} and Arrowood, {Michael J.}",
year = "2000",
month = "6",
language = "English (US)",
volume = "86",
pages = "577--582",
journal = "Journal of Parasitology",
issn = "0022-3395",
publisher = "American Society of Parasitologists",
number = "3",

}

TY - JOUR

T1 - Attempts to establish experimental Cyclospora cayetanensis infection in laboratory animals

AU - Eberhard, Mark L.

AU - Ortega, Ynes R.

AU - Hanes, Darcy E.

AU - Nace, Eva K.

AU - Do, Rose Quy

AU - Robl, Martin G.

AU - Won, Kimberly Y.

AU - Gavidia, Cesar

AU - Sass, Neil L.

AU - Mansfield, Keith

AU - Gozalo, Alfonso

AU - Griffiths, Jeffrey

AU - Gilman, Robert

AU - Sterling, Charles R

AU - Arrowood, Michael J.

PY - 2000/6

Y1 - 2000/6

N2 - Attempts were made to develop an animal model for Cyclospora cayetanensis to identify a practical laboratory host for studying human cyclosporiasis. Oocysts collected from stool of infected humans in the United States, Haiti, Guatemala, Peru, and Nepal were held in potassium dichromate solution to allow development of sporozoites. The following animal types were inoculated: 9 strains of mice, including adult and neonatal immunocompetent and immune-deficient inbred and outbred strains, rats, sandrats, chickens, ducks, rabbits, jirds, hamsters, ferrets, pigs, dogs, owl monkeys, rhesus monkeys, and cynomolgus monkeys. Most animals were inoculated by gavage, although some of the primates were fed oocysts on food items. The animals were examined for signs of infection, particularly diarrhea, and stool samples were examined for 4-6 wk after inoculation. None of the animals developed patent infections or signs of infection. We conclude that none of the animals tested is susceptible to infection with C. cayetanensis.

AB - Attempts were made to develop an animal model for Cyclospora cayetanensis to identify a practical laboratory host for studying human cyclosporiasis. Oocysts collected from stool of infected humans in the United States, Haiti, Guatemala, Peru, and Nepal were held in potassium dichromate solution to allow development of sporozoites. The following animal types were inoculated: 9 strains of mice, including adult and neonatal immunocompetent and immune-deficient inbred and outbred strains, rats, sandrats, chickens, ducks, rabbits, jirds, hamsters, ferrets, pigs, dogs, owl monkeys, rhesus monkeys, and cynomolgus monkeys. Most animals were inoculated by gavage, although some of the primates were fed oocysts on food items. The animals were examined for signs of infection, particularly diarrhea, and stool samples were examined for 4-6 wk after inoculation. None of the animals developed patent infections or signs of infection. We conclude that none of the animals tested is susceptible to infection with C. cayetanensis.

UR - http://www.scopus.com/inward/record.url?scp=0033917204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033917204&partnerID=8YFLogxK

M3 - Article

C2 - 10864257

AN - SCOPUS:0033917204

VL - 86

SP - 577

EP - 582

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

IS - 3

ER -