Attenuation of gastrin-induced gastric acid secretion by antisense oligonucleotide to the CCK(B)/gastrin receptor

R. K. Rao, Y. Lopez, J. Lai, P. J M Riviere, J. L. Junien, Frank Porreca

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effects of treatment with CCK receptor antagonists or administration of an antisense oligonucleotide to the gastrin receptor, on gastrin-I and cholecystokinin-8-induced acid secretion in mouse stomach were evaluated. Administration of gastrin-I (1 μM) or cholecystokinin-8 (30 nM) stimulated acid output at the rates of 2.6 ± 0.27 and 1.0 ± 0.21 μEq h-1, respectively. Gastrin-I-induced acid output was significantly blocked by pretreatment of stomachs with 3R[+]-N-[2,3-dihydro-1-methyl-2-oxo-5-phenyl-1H-1,4-benzodiazepin-3-yl ]-N[3-methlyphenyl]urea (L-365,260; 1 μM), but not by devazepide (L-364,718; 1 μM). Cholecystokinin-8-induced acid output, on the other hand, was sensitive to both L-365,260 (100 nM) and L-364,718 (100 nM). Administration of antisense, but not mismatch, oligonucleotide significantly reduced gastrin-induced acid output, while antisense oligonucleotide treatment had no effect on inin-8-induced acid output. These results of antagonist and antisense oligonucleotide studies suggest that gastrin-I and cholecystokinin-8 may involve different receptor subtypes in stimulating gastric acid secretion in mice, and that antisense oligonucleotide administration may serve as a useful tool in characterizing CCK/gastrin receptor subtypes.

Original languageEnglish (US)
Pages (from-to)2373-2377
Number of pages5
JournalNeuroReport
Volume6
Issue number17
StatePublished - 1995

Fingerprint

Cholecystokinin B Receptor
Antisense Oligonucleotides
Gastric Acid
Gastrins
Devazepide
Acids
Cholecystokinin Receptors
Stomach
Oligonucleotides
Urea
cholecystokinin 8
gastrin I

Keywords

  • cholecystokinin
  • gastric secretion
  • gastrin
  • receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Rao, R. K., Lopez, Y., Lai, J., Riviere, P. J. M., Junien, J. L., & Porreca, F. (1995). Attenuation of gastrin-induced gastric acid secretion by antisense oligonucleotide to the CCK(B)/gastrin receptor. NeuroReport, 6(17), 2373-2377.

Attenuation of gastrin-induced gastric acid secretion by antisense oligonucleotide to the CCK(B)/gastrin receptor. / Rao, R. K.; Lopez, Y.; Lai, J.; Riviere, P. J M; Junien, J. L.; Porreca, Frank.

In: NeuroReport, Vol. 6, No. 17, 1995, p. 2373-2377.

Research output: Contribution to journalArticle

Rao, RK, Lopez, Y, Lai, J, Riviere, PJM, Junien, JL & Porreca, F 1995, 'Attenuation of gastrin-induced gastric acid secretion by antisense oligonucleotide to the CCK(B)/gastrin receptor', NeuroReport, vol. 6, no. 17, pp. 2373-2377.
Rao, R. K. ; Lopez, Y. ; Lai, J. ; Riviere, P. J M ; Junien, J. L. ; Porreca, Frank. / Attenuation of gastrin-induced gastric acid secretion by antisense oligonucleotide to the CCK(B)/gastrin receptor. In: NeuroReport. 1995 ; Vol. 6, No. 17. pp. 2373-2377.
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