Aurora-A over-expression in high-grade PIN lesions and prostate cancer

Holly McKlveen Buschhorn, Robert R. Klein, Susan M. Chambers, Margaret C. Hardy, Sylvan Green, David Bearss, Raymond B Nagle

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

BACKGROUND. Over-expression of Aurora-A (Aurora 2 kinase, STK-15), a protein found in centrosomes thought to be associated with genetic instability, has been previously documented in prostate cancer [Pihan et al.: Cancer Res 61(5):2212-2219, 2001]. It is unknown if this protein is also over-expressed in high-grade prostatic intraepithelial neoplasia (PIN) lesions. METHODS. PIN lesions were examined for increased Aurora-A using immunohistochemical staining on archival paraffin embedded prostatectomy tissue. Aurora-A expression was scored using size, number, and staining intensity. Protein expression was examined and compared between stromal cells, normal glands, high-grade PIN lesions, and invasive cancer. RESULTS. Immunohistochemistry shows an increased expression of Aurora-A in 96% of high-grade PIN cases, and 98% in cancer lesions. Twenty-nine percent of cases of normal glands from cancerous prostates also showed increased Aurora-A expression. CONCLUSIONS. Over-expression of Aurora-A is present in some normal and the majority of high-grade PIN lesions indicating that this may be an early event that leads to the genetic instability seen in prostate carcinogenesis.

Original languageEnglish (US)
Pages (from-to)341-346
Number of pages6
JournalProstate
Volume64
Issue number4
DOIs
StatePublished - Sep 1 2005

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Prostatic Intraepithelial Neoplasia
Prostatic Neoplasms
Prostate
Aurora Kinase A
Staining and Labeling
Neoplasms
Centrosome
Proteins
Stromal Cells
Prostatectomy
Paraffin
Carcinogenesis
Immunohistochemistry

Keywords

  • Aurora-A
  • Centrosomes
  • Genetic instability
  • Immunohistochemistry
  • Prostatic intraepithelial neoplasia

ASJC Scopus subject areas

  • Urology

Cite this

Buschhorn, H. M., Klein, R. R., Chambers, S. M., Hardy, M. C., Green, S., Bearss, D., & Nagle, R. B. (2005). Aurora-A over-expression in high-grade PIN lesions and prostate cancer. Prostate, 64(4), 341-346. https://doi.org/10.1002/pros.20247

Aurora-A over-expression in high-grade PIN lesions and prostate cancer. / Buschhorn, Holly McKlveen; Klein, Robert R.; Chambers, Susan M.; Hardy, Margaret C.; Green, Sylvan; Bearss, David; Nagle, Raymond B.

In: Prostate, Vol. 64, No. 4, 01.09.2005, p. 341-346.

Research output: Contribution to journalArticle

Buschhorn, HM, Klein, RR, Chambers, SM, Hardy, MC, Green, S, Bearss, D & Nagle, RB 2005, 'Aurora-A over-expression in high-grade PIN lesions and prostate cancer', Prostate, vol. 64, no. 4, pp. 341-346. https://doi.org/10.1002/pros.20247
Buschhorn HM, Klein RR, Chambers SM, Hardy MC, Green S, Bearss D et al. Aurora-A over-expression in high-grade PIN lesions and prostate cancer. Prostate. 2005 Sep 1;64(4):341-346. https://doi.org/10.1002/pros.20247
Buschhorn, Holly McKlveen ; Klein, Robert R. ; Chambers, Susan M. ; Hardy, Margaret C. ; Green, Sylvan ; Bearss, David ; Nagle, Raymond B. / Aurora-A over-expression in high-grade PIN lesions and prostate cancer. In: Prostate. 2005 ; Vol. 64, No. 4. pp. 341-346.
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N2 - BACKGROUND. Over-expression of Aurora-A (Aurora 2 kinase, STK-15), a protein found in centrosomes thought to be associated with genetic instability, has been previously documented in prostate cancer [Pihan et al.: Cancer Res 61(5):2212-2219, 2001]. It is unknown if this protein is also over-expressed in high-grade prostatic intraepithelial neoplasia (PIN) lesions. METHODS. PIN lesions were examined for increased Aurora-A using immunohistochemical staining on archival paraffin embedded prostatectomy tissue. Aurora-A expression was scored using size, number, and staining intensity. Protein expression was examined and compared between stromal cells, normal glands, high-grade PIN lesions, and invasive cancer. RESULTS. Immunohistochemistry shows an increased expression of Aurora-A in 96% of high-grade PIN cases, and 98% in cancer lesions. Twenty-nine percent of cases of normal glands from cancerous prostates also showed increased Aurora-A expression. CONCLUSIONS. Over-expression of Aurora-A is present in some normal and the majority of high-grade PIN lesions indicating that this may be an early event that leads to the genetic instability seen in prostate carcinogenesis.

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