Autoclaving as a chemical-free process to stabilize recombinant silk-elastinlike protein polymer nanofibers

Weiguo Qiu, Joseph Cappello, Xiaoyi Wu

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Abstract

We report here that autoclaving is a chemical-free, physical crosslinking strategy capable of stabilizing electrospun recombinant silk-elastinlike protein (SELP) polymer nanofibers. Fourier transform infrared spectroscopy showed that the autoclaving of SELP nanofibers induced a conformational conversion of -turns and unordered structures to ordered -sheets. Tensile stress-strain analysis of the autoclaved SELP nanofibrous scaffolds in phosphate buffered saline at 37 °C revealed a Young's modulus of 1.02 ±0.28 MPa, an ultimate tensile strength of 0.34 ±0.04 MPa, and a strain at failure of 29% ±3%.

Original languageEnglish (US)
Article number263702
JournalApplied Physics Letters
Volume98
Issue number26
DOIs
StatePublished - Jun 27 2011

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ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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