Autoclaving as a chemical-free process to stabilize recombinant silk-elastinlike protein polymer nanofibers

Weiguo Qiu, Joseph Cappello, Xiaoyi Wu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We report here that autoclaving is a chemical-free, physical crosslinking strategy capable of stabilizing electrospun recombinant silk-elastinlike protein (SELP) polymer nanofibers. Fourier transform infrared spectroscopy showed that the autoclaving of SELP nanofibers induced a conformational conversion of -turns and unordered structures to ordered -sheets. Tensile stress-strain analysis of the autoclaved SELP nanofibrous scaffolds in phosphate buffered saline at 37 °C revealed a Young's modulus of 1.02 ±0.28 MPa, an ultimate tensile strength of 0.34 ±0.04 MPa, and a strain at failure of 29% ±3%.

Original languageEnglish (US)
Article number263702
JournalApplied Physics Letters
Volume98
Issue number26
DOIs
StatePublished - Jun 27 2011

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autoclaving
silk
proteins
polymers
crosslinking
tensile stress
tensile strength
modulus of elasticity
phosphates
infrared spectroscopy

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Autoclaving as a chemical-free process to stabilize recombinant silk-elastinlike protein polymer nanofibers. / Qiu, Weiguo; Cappello, Joseph; Wu, Xiaoyi.

In: Applied Physics Letters, Vol. 98, No. 26, 263702, 27.06.2011.

Research output: Contribution to journalArticle

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