We report here that autoclaving is a chemical-free, physical crosslinking strategy capable of stabilizing electrospun recombinant silk-elastinlike protein (SELP) polymer nanofibers. Fourier transform infrared spectroscopy showed that the autoclaving of SELP nanofibers induced a conformational conversion of -turns and unordered structures to ordered -sheets. Tensile stress-strain analysis of the autoclaved SELP nanofibrous scaffolds in phosphate buffered saline at 37 °C revealed a Young's modulus of 1.02 ±0.28 MPa, an ultimate tensile strength of 0.34 ±0.04 MPa, and a strain at failure of 29% ±3%.
ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)