Autophagy correlates with maintenance of salivary gland function following radiation

Maria Morgan-Bathke, Grace A. Hill, Zoey I. Harris, Her H. Lin, Alex M. Chibly, Rob R. Klein, Randy M Burd, David K. Ann, Kirsten Limesand

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The current standard of care for head and neck cancer includes surgical resection of the tumor followed by targeted head and neck radiation. This radiotherapy results in a multitude of negative side effects in adjacent normal tissues. Autophagy is a cellular mechanism that could be targeted to ameliorate these side effects based on its role in cellular homeostasis. In this study, we utilized Atg5 f/f;Aqp5-Cre mice which harbor a conditional knockout of Atg5, in salivary acinar cells. These autophagy-deficient mice display increased radiosensitivity. Treatment of wild-type mice with radiation did not robustly induce autophagy following radiotherapy, however, using a model of preserved salivary gland function by IGF-1-treatment prior to irradiation, we demonstrate increased autophagosome formation 6-8 hours following radiation. Additionally, administration of IGF-1 to Atg5 f/f;Aqp5-Cre mice did not preserve physiological function. Thus, autophagy appears to play a beneficial role in salivary glands following radiation and pharmacological induction of autophagy could alleviate the negative side effects associated with therapy for head and neck cancer.

Original languageEnglish (US)
Article number5206
JournalScientific Reports
Volume4
DOIs
StatePublished - Jun 6 2014

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Autophagy
Salivary Glands
Maintenance
Radiation
Head and Neck Neoplasms
Insulin-Like Growth Factor I
Radiotherapy
Acinar Cells
Radiation Tolerance
Standard of Care
Homeostasis
Neck
Therapeutics
Head
Pharmacology
Neoplasms

ASJC Scopus subject areas

  • General

Cite this

Morgan-Bathke, M., Hill, G. A., Harris, Z. I., Lin, H. H., Chibly, A. M., Klein, R. R., ... Limesand, K. (2014). Autophagy correlates with maintenance of salivary gland function following radiation. Scientific Reports, 4, [5206]. https://doi.org/10.1038/srep05206

Autophagy correlates with maintenance of salivary gland function following radiation. / Morgan-Bathke, Maria; Hill, Grace A.; Harris, Zoey I.; Lin, Her H.; Chibly, Alex M.; Klein, Rob R.; Burd, Randy M; Ann, David K.; Limesand, Kirsten.

In: Scientific Reports, Vol. 4, 5206, 06.06.2014.

Research output: Contribution to journalArticle

Morgan-Bathke, M, Hill, GA, Harris, ZI, Lin, HH, Chibly, AM, Klein, RR, Burd, RM, Ann, DK & Limesand, K 2014, 'Autophagy correlates with maintenance of salivary gland function following radiation', Scientific Reports, vol. 4, 5206. https://doi.org/10.1038/srep05206
Morgan-Bathke M, Hill GA, Harris ZI, Lin HH, Chibly AM, Klein RR et al. Autophagy correlates with maintenance of salivary gland function following radiation. Scientific Reports. 2014 Jun 6;4. 5206. https://doi.org/10.1038/srep05206
Morgan-Bathke, Maria ; Hill, Grace A. ; Harris, Zoey I. ; Lin, Her H. ; Chibly, Alex M. ; Klein, Rob R. ; Burd, Randy M ; Ann, David K. ; Limesand, Kirsten. / Autophagy correlates with maintenance of salivary gland function following radiation. In: Scientific Reports. 2014 ; Vol. 4.
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