Autophagy is the predominant process induced by arsenite in human lymphoblastoid cell lines

Alicia M. Bolt, Randi M. Byrd, Walter Klimecki

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Arsenic is a widespread environmental toxicant with a diverse array of molecular targets and associated diseases, making the identification of the critical mechanisms and pathways of arsenic-induced cytotoxicity a challenge. In a variety of experimental models, over a range of arsenic exposure levels, apoptosis is a commonly identified arsenic-induced cytotoxic pathway. Human lymphoblastoid cell lines (LCL) have been used as a model system in arsenic toxicology for many years, but the exact mechanism of arsenic-induced cytotoxicity in LCL is still unknown. We investigated the cytotoxicity of sodium arsenite in LCL 18564 using a set of complementary markers for cell death pathways. Markers indicative of apoptosis (phosphatidylserine externalization, PARP cleavage, and sensitivity to caspase inhibition) were uniformly negative in arsenite exposed cells. Interestingly, electron microscopy, acidic vesicle fluorescence, and expression of LC3 in LCL 18564 identified autophagy as an arsenite-induced process that was associated with cytotoxicity. Autophagy, a cellular programmed response that is associated with both cellular stress adaptation as well as cell death appears to be the predominant process in LCL cytotoxicity induced by arsenite. It is unclear, however, whether LCL autophagy is an effector mechanism of arsenite cytotoxicity or alternatively a cellular compensatory mechanism. The ability of arsenite to induce autophagy in lymphoblastoid cell lines introduces a potentially novel mechanistic explanation of the well-characterized in vitro and in vivo toxicity of arsenic to lymphoid cells.

Original languageEnglish (US)
Pages (from-to)366-373
Number of pages8
JournalToxicology and Applied Pharmacology
Volume244
Issue number3
DOIs
StatePublished - May 2010

Fingerprint

Autophagy
Arsenic
Cytotoxicity
Cells
Cell Line
Cell death
Cell Death
Apoptosis
Critical Pathways
Phosphatidylserines
Caspases
arsenite
Toxicology
Electron microscopy
Toxicity
Electron Microscopy
Theoretical Models
Fluorescence
Lymphocytes

Keywords

  • Autophagy
  • Cytotoxicity
  • Lymphoblastoid
  • Sodium arsenite

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Autophagy is the predominant process induced by arsenite in human lymphoblastoid cell lines. / Bolt, Alicia M.; Byrd, Randi M.; Klimecki, Walter.

In: Toxicology and Applied Pharmacology, Vol. 244, No. 3, 05.2010, p. 366-373.

Research output: Contribution to journalArticle

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