Autoradiographic analysis of amino acid uptake by the gill of Mytilus

Stephen Wright, Kathleen M. Southwell, Grover C. Stephens

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Autoradiography was used to examine the influence of lateral ciliary activity on the pattern of leucine uptake into isolated gill tissue from the mussel, Mytilus californianus. Metachronal activity of the lateral cilia, normally absent in the in vitro gill, was reestablished by application of 10 μM 5-hydroxytryptamine (5-HT). This treatment produced a 5-7 fold stimulation in the rate of leucine uptake into isolated gills. The treatment with 5-HT did not, however, affect the fractional incorporation of leucine into alcohol insoluble vs alcohol soluble material. Autoradiograms of gills treated with 5-HT showed extensive labelling of frontal, lateral, and abfrontal surfaces of gill filaments compared to the control condition in which label was largely confined to the frontal region of the gill. Quantitative analyses of the autoradiograms revealed a 4-fold increase in the number of silver grains over lateral and abfrontal surfaces compared to control gills. Autoradiograms of gills from intact mussels exposed to3H-leucine showed a pattern of silver grain deposition similar to that observed in in vitro gills treated with 5-HT. It is concluded that the capacity for amino acid transport exists in cells from the frontal, lateral, and abfrontal surfaces of gill filaments, butaccess to dissolved substrates by transport sites on lateral and abfrontal surfaces is dependent upon lateral ciliary activity.

Original languageEnglish (US)
Pages (from-to)249-256
Number of pages8
JournalJournal of Comparative Physiology B
Volume154
Issue number3
DOIs
StatePublished - Apr 1984
Externally publishedYes

Fingerprint

Mytilus
Leucine
Serotonin
gills
amino acid
uptake mechanisms
Amino Acids
amino acids
Silver
alcohol
silver
serotonin
Alcohols
leucine
fold
Labeling
Bivalvia
Labels
mussels
Tissue

ASJC Scopus subject areas

  • Physiology (medical)
  • Environmental Science(all)
  • Physiology
  • Animal Science and Zoology

Cite this

Autoradiographic analysis of amino acid uptake by the gill of Mytilus. / Wright, Stephen; Southwell, Kathleen M.; Stephens, Grover C.

In: Journal of Comparative Physiology B, Vol. 154, No. 3, 04.1984, p. 249-256.

Research output: Contribution to journalArticle

Wright, Stephen ; Southwell, Kathleen M. ; Stephens, Grover C. / Autoradiographic analysis of amino acid uptake by the gill of Mytilus. In: Journal of Comparative Physiology B. 1984 ; Vol. 154, No. 3. pp. 249-256.
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