Autoradiographic localization of muscarinic agonist binding sites in the rat central nervous system with (+)-cis-[3H]methyldioxolane

Henry I. Yamamura, Thomas W. Vickroy, Donald R. Gehlert, James K. Wamsley, William R. Roeske

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

(+)-cis-[3H]Methyldioxolane ((+)-[3H]CD), a potent muscarinic agonist, was used to label high-affinity agonist states of muscarinic receptors in thin tissue sections of the rat central nervous system. Light microscopic autoradiography of atropine-sensitive (+)-[3H]CD binding sites revealed regions of dense labeling (superior colliculus, inferior colliculus, lateral geniculate body, hypoglossal (XII) nucleus, facial (VII) nucleus, tractus diagonalis) and regions of sparse labeling (hippocampus, dentate gyrus). The inverse regional correlation between high-affinity (+)-[3H]CD states and binding sites for the muscarinic antagonists [3H]pirenzepine (r = -0.79) and (-)-[3H]quinuclidinyl benzilate (r = -0.30) underscores potentially important differences between agonist and antagonist binding to CNS tissue slices.

Original languageEnglish (US)
Pages (from-to)340-344
Number of pages5
JournalBrain Research
Volume325
Issue number1-2
DOIs
StatePublished - Jan 28 1985
Externally publishedYes

Keywords

  • (+)-cis-[H]methyldioxolane (+)-[H]CD
  • high-affinity agonist state
  • light microscopic autoradiography
  • muscarinic agonists

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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