Bacterial and archaeal community structure of two adjacent calcite speleothems in Kartchner Caverns, Arizona, USA

Antje Legatzki, Marian Ortiz, Julia W Neilson, Sky Dominguez, Gary L. Andersen, Rickard S. Toomey, Barry M Pryor, Leland S. Pierson, Raina Margaret Maier

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Information concerning the bacterial and archaeal communities present on calcite speleothems in carbonate caves is of interest because the activity of these microbes has been implicated as a potential biogenic component in the formation of secondary mineral deposits. In addition, these speleothems may harbor unique, previously unidentified microbes. The current study presents a comparative analysis of the superficial bacterial and archaeal community structure of multiple stalactites from two different cave formations located in close proximity to each other in a nonhuman-impacted area of Kartchner Caverns, Arizona, USA. PCR-denaturing gradient gel electrophoresis analysis (PCR-DGGE) revealed that microbial communities sampled from stalactites of a single speleothem are more similar to each other than to the communities sampled from stalactites of an adjacent speleothem, suggesting that both bacterial and archaeal communities are speleothem-specific. SR-XRD analysis confirmed that both speleothems sampled were primarily calcite, but subtle differences were detected in the elemental composition profiles obtained from ICP-MS analysis indicating that substrate geochemistry was also speleothem-specific. PhyloChip analysis of composite samples from both speleothems revealed a broad diversity of phyla represented in the bacterial communities, while bacterial and archaeal bands sequenced from the DGGE profiles confirmed the presence of unique phylotypes not closely related (< 96% similarity) to any sequences deposited in the GenBank database.

Original languageEnglish (US)
Pages (from-to)99-117
Number of pages19
JournalGeomicrobiology Journal
Volume28
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

Caves
speleothem
Calcium Carbonate
cavern
community structure
calcite
Mineral resources
Geochemistry
Carbonates
Ports and harbors
Electrophoresis
Denaturing Gradient Gel Electrophoresis
Gels
Nucleic Acid Databases
Minerals
Composite materials
Substrates
Chemical analysis
Databases
cave

Keywords

  • Carbonate cave
  • Community structure
  • DGGE
  • Phylochip
  • Speleothem

ASJC Scopus subject areas

  • Earth and Planetary Sciences (miscellaneous)
  • Microbiology
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Bacterial and archaeal community structure of two adjacent calcite speleothems in Kartchner Caverns, Arizona, USA. / Legatzki, Antje; Ortiz, Marian; Neilson, Julia W; Dominguez, Sky; Andersen, Gary L.; Toomey, Rickard S.; Pryor, Barry M; Pierson, Leland S.; Maier, Raina Margaret.

In: Geomicrobiology Journal, Vol. 28, No. 2, 02.2011, p. 99-117.

Research output: Contribution to journalArticle

Legatzki, Antje ; Ortiz, Marian ; Neilson, Julia W ; Dominguez, Sky ; Andersen, Gary L. ; Toomey, Rickard S. ; Pryor, Barry M ; Pierson, Leland S. ; Maier, Raina Margaret. / Bacterial and archaeal community structure of two adjacent calcite speleothems in Kartchner Caverns, Arizona, USA. In: Geomicrobiology Journal. 2011 ; Vol. 28, No. 2. pp. 99-117.
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