Bacterial endosymbionts from the genus Camponotus (Hymenoptera: Formicidae)

John J. Peloquin, Stephen G. Miller, Stephen A Klotz, Richard Stouthammer, Lloyd R. Davis, John H. Klotz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Gram-negative prokaryotic endosymbionts from follicle cells of eight species of North American Camponotus were isolated and their 16S rRNA-encoding DNA was amplified, cloned and sequenced. A BLASTn similarity search showed that the endosymbiont of Camponotus floridanus is most similar to Candidatus camponotii. Other than this ant endosymbiont, the most similar sequences were from various enterobacteria commonly found in the gut of insects and other animals. The most parsimonious phylogenetic tree generated by PAUP analysis on manually aligned sequences supports a monophyletic relationship of the ant endosymbionts when the 16S RNA sequences of two other insect-associated enterobacteria (Yersinia pestis, and Buchnera aphidicola) are included. The most parsimonious tree does not support, however, a monophyletic grouping of endosymbionts of the North American species when Old World isolates of Camponotus-associated eubacterial sequences are added to the analysis. These results suggest that the association and divergence of the endosymbiont with their ant host is ancient.

Original languageEnglish (US)
Pages (from-to)695-708
Number of pages14
JournalSociobiology
Volume38
Issue number3 B
StatePublished - 2001
Externally publishedYes

Fingerprint

Camponotus
endosymbionts
Formicidae
Hymenoptera
Camponotus floridanus
Buchnera aphidicola
Yersinia pestis
insects
digestive system
ribosomal RNA
nucleotide sequences
phylogeny
DNA
animals

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Insect Science

Cite this

Peloquin, J. J., Miller, S. G., Klotz, S. A., Stouthammer, R., Davis, L. R., & Klotz, J. H. (2001). Bacterial endosymbionts from the genus Camponotus (Hymenoptera: Formicidae). Sociobiology, 38(3 B), 695-708.

Bacterial endosymbionts from the genus Camponotus (Hymenoptera : Formicidae). / Peloquin, John J.; Miller, Stephen G.; Klotz, Stephen A; Stouthammer, Richard; Davis, Lloyd R.; Klotz, John H.

In: Sociobiology, Vol. 38, No. 3 B, 2001, p. 695-708.

Research output: Contribution to journalArticle

Peloquin, JJ, Miller, SG, Klotz, SA, Stouthammer, R, Davis, LR & Klotz, JH 2001, 'Bacterial endosymbionts from the genus Camponotus (Hymenoptera: Formicidae)', Sociobiology, vol. 38, no. 3 B, pp. 695-708.
Peloquin JJ, Miller SG, Klotz SA, Stouthammer R, Davis LR, Klotz JH. Bacterial endosymbionts from the genus Camponotus (Hymenoptera: Formicidae). Sociobiology. 2001;38(3 B):695-708.
Peloquin, John J. ; Miller, Stephen G. ; Klotz, Stephen A ; Stouthammer, Richard ; Davis, Lloyd R. ; Klotz, John H. / Bacterial endosymbionts from the genus Camponotus (Hymenoptera : Formicidae). In: Sociobiology. 2001 ; Vol. 38, No. 3 B. pp. 695-708.
@article{28839199141f44fca2f51cc5ee76cf78,
title = "Bacterial endosymbionts from the genus Camponotus (Hymenoptera: Formicidae)",
abstract = "Gram-negative prokaryotic endosymbionts from follicle cells of eight species of North American Camponotus were isolated and their 16S rRNA-encoding DNA was amplified, cloned and sequenced. A BLASTn similarity search showed that the endosymbiont of Camponotus floridanus is most similar to Candidatus camponotii. Other than this ant endosymbiont, the most similar sequences were from various enterobacteria commonly found in the gut of insects and other animals. The most parsimonious phylogenetic tree generated by PAUP analysis on manually aligned sequences supports a monophyletic relationship of the ant endosymbionts when the 16S RNA sequences of two other insect-associated enterobacteria (Yersinia pestis, and Buchnera aphidicola) are included. The most parsimonious tree does not support, however, a monophyletic grouping of endosymbionts of the North American species when Old World isolates of Camponotus-associated eubacterial sequences are added to the analysis. These results suggest that the association and divergence of the endosymbiont with their ant host is ancient.",
author = "Peloquin, {John J.} and Miller, {Stephen G.} and Klotz, {Stephen A} and Richard Stouthammer and Davis, {Lloyd R.} and Klotz, {John H.}",
year = "2001",
language = "English (US)",
volume = "38",
pages = "695--708",
journal = "Sociobiology",
issn = "0361-6525",
publisher = "California State University, Chico",
number = "3 B",

}

TY - JOUR

T1 - Bacterial endosymbionts from the genus Camponotus (Hymenoptera

T2 - Formicidae)

AU - Peloquin, John J.

AU - Miller, Stephen G.

AU - Klotz, Stephen A

AU - Stouthammer, Richard

AU - Davis, Lloyd R.

AU - Klotz, John H.

PY - 2001

Y1 - 2001

N2 - Gram-negative prokaryotic endosymbionts from follicle cells of eight species of North American Camponotus were isolated and their 16S rRNA-encoding DNA was amplified, cloned and sequenced. A BLASTn similarity search showed that the endosymbiont of Camponotus floridanus is most similar to Candidatus camponotii. Other than this ant endosymbiont, the most similar sequences were from various enterobacteria commonly found in the gut of insects and other animals. The most parsimonious phylogenetic tree generated by PAUP analysis on manually aligned sequences supports a monophyletic relationship of the ant endosymbionts when the 16S RNA sequences of two other insect-associated enterobacteria (Yersinia pestis, and Buchnera aphidicola) are included. The most parsimonious tree does not support, however, a monophyletic grouping of endosymbionts of the North American species when Old World isolates of Camponotus-associated eubacterial sequences are added to the analysis. These results suggest that the association and divergence of the endosymbiont with their ant host is ancient.

AB - Gram-negative prokaryotic endosymbionts from follicle cells of eight species of North American Camponotus were isolated and their 16S rRNA-encoding DNA was amplified, cloned and sequenced. A BLASTn similarity search showed that the endosymbiont of Camponotus floridanus is most similar to Candidatus camponotii. Other than this ant endosymbiont, the most similar sequences were from various enterobacteria commonly found in the gut of insects and other animals. The most parsimonious phylogenetic tree generated by PAUP analysis on manually aligned sequences supports a monophyletic relationship of the ant endosymbionts when the 16S RNA sequences of two other insect-associated enterobacteria (Yersinia pestis, and Buchnera aphidicola) are included. The most parsimonious tree does not support, however, a monophyletic grouping of endosymbionts of the North American species when Old World isolates of Camponotus-associated eubacterial sequences are added to the analysis. These results suggest that the association and divergence of the endosymbiont with their ant host is ancient.

UR - http://www.scopus.com/inward/record.url?scp=0035598044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035598044&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0035598044

VL - 38

SP - 695

EP - 708

JO - Sociobiology

JF - Sociobiology

SN - 0361-6525

IS - 3 B

ER -