Bacterial hand contamination and transfer after use of contaminated bulk-soap-refillable dispensers

Carrie A. Zapka, Esther J. Campbell, Sheri L. Maxwell, Charles P Gerba, Michael J. Dolan, James W. Arbogast, David R. Macinga

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Bulk-soap-refillable dispensers are prone to extrinsic bacterial contamination, and recent studies demonstrated that approximately one in four dispensers in public restrooms are contaminated. The purpose of this study was to quantify bacterial hand contamination and transfer after use of contaminated soap under controlled laboratory and in-use conditions in a community setting. Under laboratory conditions using liquid soap experimentally contaminated with 7.51 log10 CFU/ml of Serratia marcescens, an average of 5.28 log10 CFU remained on each hand after washing, and 2.23 log10 CFU was transferred to an agar surface. In an elementary-school-based field study, Gram-negative bacteria on the hands of students and staff increased by 1.42 log10 CFU per hand (26-fold) after washing with soap from contaminated bulk-soap-refillable dispensers. In contrast, washing with soap from dispensers with sealed refills significantly reduced bacteria on hands by 0.30 log10 CFU per hand (2-fold). Additionally, the mean number of Gram-negative bacteria transferred to surfaces after washing with soap from dispensers with sealed-soap refills (0.06 log10 CFU) was significantly lower than the mean number after washing with contaminated bulk-soap-refillable dispensers (0.74 log10 CFU; P < 0.01). Finally, significantly higher levels of Gram-negative bacteria were recovered from students (2.82 log10 CFU per hand) than were recovered from staff (2.22 log10 CFU per hand) after washing with contaminated bulk soap (P < 0.01). These results demonstrate that washing with contaminated soap from bulk-soaprefillable dispensers can increase the number of opportunistic pathogens on the hands and may play a role in the transmission of bacteria in public settings.

Original languageEnglish (US)
Pages (from-to)2898-2904
Number of pages7
JournalApplied and Environmental Microbiology
Volume77
Issue number9
DOIs
StatePublished - May 2011

Fingerprint

Soaps
dispensers
soaps
hands
Hand
washing
bacterium
Gram-Negative Bacteria
Gram-negative bacteria
Hand Disinfection
students
contamination
soap
student
Students
fold
Bacteria
Serratia marcescens
elementary schools
bacterial contamination

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Zapka, C. A., Campbell, E. J., Maxwell, S. L., Gerba, C. P., Dolan, M. J., Arbogast, J. W., & Macinga, D. R. (2011). Bacterial hand contamination and transfer after use of contaminated bulk-soap-refillable dispensers. Applied and Environmental Microbiology, 77(9), 2898-2904. https://doi.org/10.1128/AEM.02632-10

Bacterial hand contamination and transfer after use of contaminated bulk-soap-refillable dispensers. / Zapka, Carrie A.; Campbell, Esther J.; Maxwell, Sheri L.; Gerba, Charles P; Dolan, Michael J.; Arbogast, James W.; Macinga, David R.

In: Applied and Environmental Microbiology, Vol. 77, No. 9, 05.2011, p. 2898-2904.

Research output: Contribution to journalArticle

Zapka, Carrie A. ; Campbell, Esther J. ; Maxwell, Sheri L. ; Gerba, Charles P ; Dolan, Michael J. ; Arbogast, James W. ; Macinga, David R. / Bacterial hand contamination and transfer after use of contaminated bulk-soap-refillable dispensers. In: Applied and Environmental Microbiology. 2011 ; Vol. 77, No. 9. pp. 2898-2904.
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