Basolateral regulation of pH(i) in proximal tubules of avian loopless and long-looped nephrons in bicarbonate

Olga H. Brokl, Christina L. Martinez, Yung Kyu Kim, Diane E. Abbott, William H Dantzler

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Abstract

In isolated, nonperfused chicken proximal tubules from both loopless reptilian-type and long-looped mammalian-type nephrons, resting intracellular pH (pH(i)), measured with pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF), was ~7.1 under control HCO3- conditions [20 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES)/5 mM HCO3-buffered medium with pH 7.4 at 37°C] and was reduced to ~6.8 in response to NH4Cl pulse. The rate of recovery of pH(i) (dpH(i)/dt) from this level to the resting level in proximal tubules from both nephron types was (1) significantly reduced by the removal of Na+ or both Na+ and Cl- from the bath, and (2) unaffected by the removal of Cl- from the bath or the presence of a high K+ concentration or Ba2+ in the bath. In proximal tubules from long-looped mammalian-type, but not loopless reptilian-type, nephrons, dpH(i)/dt was significantly reduced by the addition of either 5-(N-ethyl-N-isopropyl) amiloride (EIPA) or 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS) to the bath. These data suggest that a Na+/H+ exchanger and most likely a Na+-dependent Cl-/HCO3- exchanger are involved in basolateral regulation of pH(i) in mammalian-type nephrons whereas none of the commonly identified basolateral acid-base transporters appear to be involved in regulation of pH(i) in reptilian-type nephrons.

Original languageEnglish (US)
Pages (from-to)174-187
Number of pages14
JournalJournal of Experimental Zoology
Volume284
Issue number2
DOIs
StatePublished - Jul 1 1999

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nephrons
proximal tubules
bicarbonates
fluorescent dyes
acids
transporters
chickens

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Basolateral regulation of pH(i) in proximal tubules of avian loopless and long-looped nephrons in bicarbonate. / Brokl, Olga H.; Martinez, Christina L.; Kim, Yung Kyu; Abbott, Diane E.; Dantzler, William H.

In: Journal of Experimental Zoology, Vol. 284, No. 2, 01.07.1999, p. 174-187.

Research output: Contribution to journalArticle

Brokl, Olga H. ; Martinez, Christina L. ; Kim, Yung Kyu ; Abbott, Diane E. ; Dantzler, William H. / Basolateral regulation of pH(i) in proximal tubules of avian loopless and long-looped nephrons in bicarbonate. In: Journal of Experimental Zoology. 1999 ; Vol. 284, No. 2. pp. 174-187.
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abstract = "In isolated, nonperfused chicken proximal tubules from both loopless reptilian-type and long-looped mammalian-type nephrons, resting intracellular pH (pH(i)), measured with pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF), was ~7.1 under control HCO3- conditions [20 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES)/5 mM HCO3-buffered medium with pH 7.4 at 37°C] and was reduced to ~6.8 in response to NH4Cl pulse. The rate of recovery of pH(i) (dpH(i)/dt) from this level to the resting level in proximal tubules from both nephron types was (1) significantly reduced by the removal of Na+ or both Na+ and Cl- from the bath, and (2) unaffected by the removal of Cl- from the bath or the presence of a high K+ concentration or Ba2+ in the bath. In proximal tubules from long-looped mammalian-type, but not loopless reptilian-type, nephrons, dpH(i)/dt was significantly reduced by the addition of either 5-(N-ethyl-N-isopropyl) amiloride (EIPA) or 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS) to the bath. These data suggest that a Na+/H+ exchanger and most likely a Na+-dependent Cl-/HCO3- exchanger are involved in basolateral regulation of pH(i) in mammalian-type nephrons whereas none of the commonly identified basolateral acid-base transporters appear to be involved in regulation of pH(i) in reptilian-type nephrons.",
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