Batch equilibrium and kinetic studies of plasmid pCI adsorption onto perfusion particles

Y. Pérez-Martínez, R. M. Montesinos-Cisneros, P. Guerrero-Germán, R. Guzman-Zamudio, A. Tejeda-Mansir

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA for third-generation vaccines. The process development requires conduct basic studies in batch format to determine the characteristic parameters of the biomolecule-adsorbent system. In this work, the equilibrium and kinetic performance of the plasmid pCI adsorption onto ion-exchange perfusive particles POROS 50 HQ was studied. The isotherm obtained was appropriately described by the Langmuir model, with the parameters q m = 29.15 mg PCI/mL of adsorbent and K d = 0.0145 mg de pCI/mL. These values show a greater capacity and more specific adsorption of pCI onto the anion-exchange (AE) perfusive particles then onto AE adsorptive membranes previously studied. The kinetics of adsorption was described adequately by a lumped resistance model using a kinetic constant of k 1 = 0.0067 mL/mg s.

Original languageEnglish (US)
Pages (from-to)196-200
Number of pages5
JournalJournal of Liquid Chromatography and Related Technologies
Volume38
Issue number2
DOIs
StatePublished - Jan 20 2015

Keywords

  • DNA vaccines
  • adsorption isotherms
  • adsorption models
  • batch adsorption kinetics
  • perfusion adsorption
  • plasmid purification

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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