BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder

Yonglin Gao, Mathew Galante, James El-Mallakh, John I. Nurnberger, Nicholas A Delamere, Zhenmin Lei, Rif S. El-Mallakh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

OBJECTIVE: To determine whether single nucleotide polymorphisms (SNPs) of the brain-derived neurotrophic factor (BDNF) that have been associated with bipolar illness are associated with physiological dysfunction. Methods: Lymphoblastoid cell lines (n=30) obtained from bipolar I individuals carrying zero, one, or two copies of a BDNF SNP associated with bipolar illness (rs12273363) were utilized. Results: Proapoptotic stressors of serum deprivation alone, or serum deprivation combined with the sodium ionophore, monensin, did not alter intracellular proBDNF. Monensin treatment increased mature-BDNF (mBDNF) protein levels (P<0.05). There were no differences related to the presence of SNP or copy number. Conclusion: rs12273363 does not appear to have functional consequences that would involve its role in bipolar illness.

Original languageEnglish (US)
Pages (from-to)253-255
Number of pages3
JournalPsychiatric Genetics
Volume22
Issue number5
DOIs
StatePublished - Oct 2012

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Brain-Derived Neurotrophic Factor
Bipolar Disorder
Single Nucleotide Polymorphism
Monensin
Cell Line
Sodium Ionophores
Nerve Growth Factors
Serum

Keywords

  • apoptosis
  • bipolar disorder
  • brain-derived neurotrophic factor
  • intracellular sodium
  • single nucleotide polymorphism

ASJC Scopus subject areas

  • Genetics(clinical)
  • Psychiatry and Mental health
  • Genetics
  • Biological Psychiatry

Cite this

BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder. / Gao, Yonglin; Galante, Mathew; El-Mallakh, James; Nurnberger, John I.; Delamere, Nicholas A; Lei, Zhenmin; El-Mallakh, Rif S.

In: Psychiatric Genetics, Vol. 22, No. 5, 10.2012, p. 253-255.

Research output: Contribution to journalArticle

Gao, Y, Galante, M, El-Mallakh, J, Nurnberger, JI, Delamere, NA, Lei, Z & El-Mallakh, RS 2012, 'BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder', Psychiatric Genetics, vol. 22, no. 5, pp. 253-255. https://doi.org/10.1097/YPG.0b013e328353ae66
Gao, Yonglin ; Galante, Mathew ; El-Mallakh, James ; Nurnberger, John I. ; Delamere, Nicholas A ; Lei, Zhenmin ; El-Mallakh, Rif S. / BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder. In: Psychiatric Genetics. 2012 ; Vol. 22, No. 5. pp. 253-255.
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AU - El-Mallakh, Rif S.

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AB - OBJECTIVE: To determine whether single nucleotide polymorphisms (SNPs) of the brain-derived neurotrophic factor (BDNF) that have been associated with bipolar illness are associated with physiological dysfunction. Methods: Lymphoblastoid cell lines (n=30) obtained from bipolar I individuals carrying zero, one, or two copies of a BDNF SNP associated with bipolar illness (rs12273363) were utilized. Results: Proapoptotic stressors of serum deprivation alone, or serum deprivation combined with the sodium ionophore, monensin, did not alter intracellular proBDNF. Monensin treatment increased mature-BDNF (mBDNF) protein levels (P<0.05). There were no differences related to the presence of SNP or copy number. Conclusion: rs12273363 does not appear to have functional consequences that would involve its role in bipolar illness.

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