BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder

Yonglin Gao, Mathew Galante, James El-Mallakh, John I. Nurnberger, Nicholas A. Delamere, Zhenmin Lei, Rif S. El-Mallakh

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

OBJECTIVE: To determine whether single nucleotide polymorphisms (SNPs) of the brain-derived neurotrophic factor (BDNF) that have been associated with bipolar illness are associated with physiological dysfunction. Methods: Lymphoblastoid cell lines (n=30) obtained from bipolar I individuals carrying zero, one, or two copies of a BDNF SNP associated with bipolar illness (rs12273363) were utilized. Results: Proapoptotic stressors of serum deprivation alone, or serum deprivation combined with the sodium ionophore, monensin, did not alter intracellular proBDNF. Monensin treatment increased mature-BDNF (mBDNF) protein levels (P<0.05). There were no differences related to the presence of SNP or copy number. Conclusion: rs12273363 does not appear to have functional consequences that would involve its role in bipolar illness.

Original languageEnglish (US)
Pages (from-to)253-255
Number of pages3
JournalPsychiatric Genetics
Volume22
Issue number5
DOIs
StatePublished - Oct 1 2012

Keywords

  • apoptosis
  • bipolar disorder
  • brain-derived neurotrophic factor
  • intracellular sodium
  • single nucleotide polymorphism

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Psychiatry and Mental health
  • Biological Psychiatry

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