Betulinic acid, derived from the desert lavender Hyptis emoryi, attenuates paclitaxel-, HIV-, and nerve injury-associated peripheral sensory neuropathy via block of N- and T-type calcium channels

Shreya S. Bellampalli, Yingshi Ji, Aubin Moutal, Song Cai, E. M.Kithsiri Wijeratne, Maria A. Gandini, Jie Yu, Aude Chefdeville, Angie Dorame, Lindsey A. Chew, Cynthia L. Madura, Shizhen Luo, Gabriella Molnar, May Khanna, John M. Streicher, Gerald W. Zamponi, Leslie Gunatilaka, Rajesh Khanna

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The Federal Pain Research Strategy recommended development of nonopioid analgesics as a top priority in its strategic plan to address the significant public health crisis and individual burden of chronic pain faced by >100 million Americans. Motivated by this challenge, a natural product extracts library was screened and identified a plant extract that targets activity of voltage-gated calcium channels. This profile is of interest as a potential treatment for neuropathic pain. The active extract derived from the desert lavender plant native to southwestern United States, when subjected to bioassay-guided fractionation, afforded 3 compounds identified as pentacyclic triterpenoids, betulinic acid (BA), oleanolic acid, and ursolic acid. Betulinic acid inhibited depolarization-evoked calcium influx in dorsal root ganglion (DRG) neurons predominantly through targeting low-voltage-gated (Cav3 or T-type) and CaV2.2 (N-type) calcium channels. Voltage-clamp electrophysiology experiments revealed a reduction of Ca, but not Na, currents in sensory neurons after BA exposure. Betulinic acid inhibited spontaneous excitatory postsynaptic currents and depolarization-evoked release of calcitonin gene-related peptide from lumbar spinal cord slices. Notably, BA did not engage human mu, delta, or kappa opioid receptors. Intrathecal administration of BA reversed mechanical allodynia in rat models of chemotherapy-induced peripheral neuropathy and HIV-associated peripheral sensory neuropathy as well as a mouse model of partial sciatic nerve ligation without effects on locomotion. The broad-spectrum biological and medicinal properties reported, including anti-HIV and anticancer activities of BA and its derivatives, position this plant-derived small molecule natural product as a potential nonopioid therapy for management of chronic pain.

Original languageEnglish (US)
Pages (from-to)117-135
Number of pages19
JournalPain
Volume160
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Hyptis
T-Type Calcium Channels
N-Type Calcium Channels
Lavandula
Peripheral Nervous System Diseases
Paclitaxel
HIV
Wounds and Injuries
Biological Products
Chronic Pain
Spinal Cord
Southwestern United States
Non-Narcotic Analgesics
Oleanolic Acid
kappa Opioid Receptor
delta Opioid Receptor
Calcitonin Gene-Related Peptide
mu Opioid Receptor
Excitatory Postsynaptic Potentials
Electrophysiology

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

Cite this

Betulinic acid, derived from the desert lavender Hyptis emoryi, attenuates paclitaxel-, HIV-, and nerve injury-associated peripheral sensory neuropathy via block of N- and T-type calcium channels. / Bellampalli, Shreya S.; Ji, Yingshi; Moutal, Aubin; Cai, Song; Wijeratne, E. M.Kithsiri; Gandini, Maria A.; Yu, Jie; Chefdeville, Aude; Dorame, Angie; Chew, Lindsey A.; Madura, Cynthia L.; Luo, Shizhen; Molnar, Gabriella; Khanna, May; Streicher, John M.; Zamponi, Gerald W.; Gunatilaka, Leslie; Khanna, Rajesh.

In: Pain, Vol. 160, No. 1, 01.01.2019, p. 117-135.

Research output: Contribution to journalArticle

Bellampalli, SS, Ji, Y, Moutal, A, Cai, S, Wijeratne, EMK, Gandini, MA, Yu, J, Chefdeville, A, Dorame, A, Chew, LA, Madura, CL, Luo, S, Molnar, G, Khanna, M, Streicher, JM, Zamponi, GW, Gunatilaka, L & Khanna, R 2019, 'Betulinic acid, derived from the desert lavender Hyptis emoryi, attenuates paclitaxel-, HIV-, and nerve injury-associated peripheral sensory neuropathy via block of N- and T-type calcium channels', Pain, vol. 160, no. 1, pp. 117-135. https://doi.org/10.1097/j.pain.0000000000001385
Bellampalli, Shreya S. ; Ji, Yingshi ; Moutal, Aubin ; Cai, Song ; Wijeratne, E. M.Kithsiri ; Gandini, Maria A. ; Yu, Jie ; Chefdeville, Aude ; Dorame, Angie ; Chew, Lindsey A. ; Madura, Cynthia L. ; Luo, Shizhen ; Molnar, Gabriella ; Khanna, May ; Streicher, John M. ; Zamponi, Gerald W. ; Gunatilaka, Leslie ; Khanna, Rajesh. / Betulinic acid, derived from the desert lavender Hyptis emoryi, attenuates paclitaxel-, HIV-, and nerve injury-associated peripheral sensory neuropathy via block of N- and T-type calcium channels. In: Pain. 2019 ; Vol. 160, No. 1. pp. 117-135.
@article{c4e85086d7f64922902ef6b08501e2ea,
title = "Betulinic acid, derived from the desert lavender Hyptis emoryi, attenuates paclitaxel-, HIV-, and nerve injury-associated peripheral sensory neuropathy via block of N- and T-type calcium channels",
abstract = "The Federal Pain Research Strategy recommended development of nonopioid analgesics as a top priority in its strategic plan to address the significant public health crisis and individual burden of chronic pain faced by >100 million Americans. Motivated by this challenge, a natural product extracts library was screened and identified a plant extract that targets activity of voltage-gated calcium channels. This profile is of interest as a potential treatment for neuropathic pain. The active extract derived from the desert lavender plant native to southwestern United States, when subjected to bioassay-guided fractionation, afforded 3 compounds identified as pentacyclic triterpenoids, betulinic acid (BA), oleanolic acid, and ursolic acid. Betulinic acid inhibited depolarization-evoked calcium influx in dorsal root ganglion (DRG) neurons predominantly through targeting low-voltage-gated (Cav3 or T-type) and CaV2.2 (N-type) calcium channels. Voltage-clamp electrophysiology experiments revealed a reduction of Ca, but not Na, currents in sensory neurons after BA exposure. Betulinic acid inhibited spontaneous excitatory postsynaptic currents and depolarization-evoked release of calcitonin gene-related peptide from lumbar spinal cord slices. Notably, BA did not engage human mu, delta, or kappa opioid receptors. Intrathecal administration of BA reversed mechanical allodynia in rat models of chemotherapy-induced peripheral neuropathy and HIV-associated peripheral sensory neuropathy as well as a mouse model of partial sciatic nerve ligation without effects on locomotion. The broad-spectrum biological and medicinal properties reported, including anti-HIV and anticancer activities of BA and its derivatives, position this plant-derived small molecule natural product as a potential nonopioid therapy for management of chronic pain.",
author = "Bellampalli, {Shreya S.} and Yingshi Ji and Aubin Moutal and Song Cai and Wijeratne, {E. M.Kithsiri} and Gandini, {Maria A.} and Jie Yu and Aude Chefdeville and Angie Dorame and Chew, {Lindsey A.} and Madura, {Cynthia L.} and Shizhen Luo and Gabriella Molnar and May Khanna and Streicher, {John M.} and Zamponi, {Gerald W.} and Leslie Gunatilaka and Rajesh Khanna",
year = "2019",
month = "1",
day = "1",
doi = "10.1097/j.pain.0000000000001385",
language = "English (US)",
volume = "160",
pages = "117--135",
journal = "Pain",
issn = "0304-3959",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Betulinic acid, derived from the desert lavender Hyptis emoryi, attenuates paclitaxel-, HIV-, and nerve injury-associated peripheral sensory neuropathy via block of N- and T-type calcium channels

AU - Bellampalli, Shreya S.

AU - Ji, Yingshi

AU - Moutal, Aubin

AU - Cai, Song

AU - Wijeratne, E. M.Kithsiri

AU - Gandini, Maria A.

AU - Yu, Jie

AU - Chefdeville, Aude

AU - Dorame, Angie

AU - Chew, Lindsey A.

AU - Madura, Cynthia L.

AU - Luo, Shizhen

AU - Molnar, Gabriella

AU - Khanna, May

AU - Streicher, John M.

AU - Zamponi, Gerald W.

AU - Gunatilaka, Leslie

AU - Khanna, Rajesh

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The Federal Pain Research Strategy recommended development of nonopioid analgesics as a top priority in its strategic plan to address the significant public health crisis and individual burden of chronic pain faced by >100 million Americans. Motivated by this challenge, a natural product extracts library was screened and identified a plant extract that targets activity of voltage-gated calcium channels. This profile is of interest as a potential treatment for neuropathic pain. The active extract derived from the desert lavender plant native to southwestern United States, when subjected to bioassay-guided fractionation, afforded 3 compounds identified as pentacyclic triterpenoids, betulinic acid (BA), oleanolic acid, and ursolic acid. Betulinic acid inhibited depolarization-evoked calcium influx in dorsal root ganglion (DRG) neurons predominantly through targeting low-voltage-gated (Cav3 or T-type) and CaV2.2 (N-type) calcium channels. Voltage-clamp electrophysiology experiments revealed a reduction of Ca, but not Na, currents in sensory neurons after BA exposure. Betulinic acid inhibited spontaneous excitatory postsynaptic currents and depolarization-evoked release of calcitonin gene-related peptide from lumbar spinal cord slices. Notably, BA did not engage human mu, delta, or kappa opioid receptors. Intrathecal administration of BA reversed mechanical allodynia in rat models of chemotherapy-induced peripheral neuropathy and HIV-associated peripheral sensory neuropathy as well as a mouse model of partial sciatic nerve ligation without effects on locomotion. The broad-spectrum biological and medicinal properties reported, including anti-HIV and anticancer activities of BA and its derivatives, position this plant-derived small molecule natural product as a potential nonopioid therapy for management of chronic pain.

AB - The Federal Pain Research Strategy recommended development of nonopioid analgesics as a top priority in its strategic plan to address the significant public health crisis and individual burden of chronic pain faced by >100 million Americans. Motivated by this challenge, a natural product extracts library was screened and identified a plant extract that targets activity of voltage-gated calcium channels. This profile is of interest as a potential treatment for neuropathic pain. The active extract derived from the desert lavender plant native to southwestern United States, when subjected to bioassay-guided fractionation, afforded 3 compounds identified as pentacyclic triterpenoids, betulinic acid (BA), oleanolic acid, and ursolic acid. Betulinic acid inhibited depolarization-evoked calcium influx in dorsal root ganglion (DRG) neurons predominantly through targeting low-voltage-gated (Cav3 or T-type) and CaV2.2 (N-type) calcium channels. Voltage-clamp electrophysiology experiments revealed a reduction of Ca, but not Na, currents in sensory neurons after BA exposure. Betulinic acid inhibited spontaneous excitatory postsynaptic currents and depolarization-evoked release of calcitonin gene-related peptide from lumbar spinal cord slices. Notably, BA did not engage human mu, delta, or kappa opioid receptors. Intrathecal administration of BA reversed mechanical allodynia in rat models of chemotherapy-induced peripheral neuropathy and HIV-associated peripheral sensory neuropathy as well as a mouse model of partial sciatic nerve ligation without effects on locomotion. The broad-spectrum biological and medicinal properties reported, including anti-HIV and anticancer activities of BA and its derivatives, position this plant-derived small molecule natural product as a potential nonopioid therapy for management of chronic pain.

UR - http://www.scopus.com/inward/record.url?scp=85059172506&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059172506&partnerID=8YFLogxK

U2 - 10.1097/j.pain.0000000000001385

DO - 10.1097/j.pain.0000000000001385

M3 - Article

C2 - 30169422

AN - SCOPUS:85059172506

VL - 160

SP - 117

EP - 135

JO - Pain

JF - Pain

SN - 0304-3959

IS - 1

ER -