Abstract
Illumina amplicon sequencing of soil in a temperate pine forest in the southeastern United States detected an abundant, nitrogen (N)-responsive fungal genotype of unknown phylogenetic affiliation. Two isolates with ribosomal sequences consistent with that genotype were subsequently obtained. Examination of records in GenBank revealed that a genetically similar fungus had been isolated previously as an endophyte of moss in a pine forest in the southwestern United States. The three isolates were characterized using morphological, genomic, and multilocus molecular data (18S, internal transcribed spacer [ITS], and 28S rRNA sequences). Phylogenetic and maximum likelihood phylogenomic reconstructions revealed that the taxon represents a novel lineage in Mucoromycotina, only preceded by Calcarisporiella, the earliest diverging lineage in the subphylum. Sequences for the novel taxon are frequently detected in environmental sequencing studies, and it is currently part of UNITE’s dynamic list of most wanted fungi. The fungus is dimorphic, grows best at room temperature, and is associated with a wide variety of bacteria. Here, a new monotypic genus, Bifiguratus, is proposed, typified by Bifiguratus adelaidae.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 363-378 |
| Number of pages | 16 |
| Journal | Mycologia |
| Volume | 109 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2017 |
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Keywords
- Bryophyte
- Dimorphic
- Endophyte
- Environmental sampling
- Mucoromycota
- Pinaceae
- Soil
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Physiology
- Molecular Biology
- Genetics
- Plant Science
- Cell Biology
Cite this
Bifiguratus adelaidae, gen. et sp. nov., a new member of Mucoromycotina in endophytic and soil-dwelling habitats. / Torres-Cruz, Terry J.; Tobias, Terri L.Billingsley; Almatruk, Maryam; Hesse, Cedar N.; Kuske, Cheryl R.; Desirò, Alessandro; Benucci, Gian Maria Niccolò; Bonito, Gregory; Stajich, Jason E.; Dunlap, Christopher; Arnold, Anne E; Porras-Alfaro, Andrea.
In: Mycologia, Vol. 109, No. 3, 2017, p. 363-378.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bifiguratus adelaidae, gen. et sp. nov., a new member of Mucoromycotina in endophytic and soil-dwelling habitats
AU - Torres-Cruz, Terry J.
AU - Tobias, Terri L.Billingsley
AU - Almatruk, Maryam
AU - Hesse, Cedar N.
AU - Kuske, Cheryl R.
AU - Desirò, Alessandro
AU - Benucci, Gian Maria Niccolò
AU - Bonito, Gregory
AU - Stajich, Jason E.
AU - Dunlap, Christopher
AU - Arnold, Anne E
AU - Porras-Alfaro, Andrea
PY - 2017
Y1 - 2017
N2 - Illumina amplicon sequencing of soil in a temperate pine forest in the southeastern United States detected an abundant, nitrogen (N)-responsive fungal genotype of unknown phylogenetic affiliation. Two isolates with ribosomal sequences consistent with that genotype were subsequently obtained. Examination of records in GenBank revealed that a genetically similar fungus had been isolated previously as an endophyte of moss in a pine forest in the southwestern United States. The three isolates were characterized using morphological, genomic, and multilocus molecular data (18S, internal transcribed spacer [ITS], and 28S rRNA sequences). Phylogenetic and maximum likelihood phylogenomic reconstructions revealed that the taxon represents a novel lineage in Mucoromycotina, only preceded by Calcarisporiella, the earliest diverging lineage in the subphylum. Sequences for the novel taxon are frequently detected in environmental sequencing studies, and it is currently part of UNITE’s dynamic list of most wanted fungi. The fungus is dimorphic, grows best at room temperature, and is associated with a wide variety of bacteria. Here, a new monotypic genus, Bifiguratus, is proposed, typified by Bifiguratus adelaidae.
AB - Illumina amplicon sequencing of soil in a temperate pine forest in the southeastern United States detected an abundant, nitrogen (N)-responsive fungal genotype of unknown phylogenetic affiliation. Two isolates with ribosomal sequences consistent with that genotype were subsequently obtained. Examination of records in GenBank revealed that a genetically similar fungus had been isolated previously as an endophyte of moss in a pine forest in the southwestern United States. The three isolates were characterized using morphological, genomic, and multilocus molecular data (18S, internal transcribed spacer [ITS], and 28S rRNA sequences). Phylogenetic and maximum likelihood phylogenomic reconstructions revealed that the taxon represents a novel lineage in Mucoromycotina, only preceded by Calcarisporiella, the earliest diverging lineage in the subphylum. Sequences for the novel taxon are frequently detected in environmental sequencing studies, and it is currently part of UNITE’s dynamic list of most wanted fungi. The fungus is dimorphic, grows best at room temperature, and is associated with a wide variety of bacteria. Here, a new monotypic genus, Bifiguratus, is proposed, typified by Bifiguratus adelaidae.
KW - Bryophyte
KW - Dimorphic
KW - Endophyte
KW - Environmental sampling
KW - Mucoromycota
KW - Pinaceae
KW - Soil
UR - http://www.scopus.com/inward/record.url?scp=85033464410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85033464410&partnerID=8YFLogxK
U2 - 10.1080/00275514.2017.1364958
DO - 10.1080/00275514.2017.1364958
M3 - Article
C2 - 28876195
AN - SCOPUS:85033464410
VL - 109
SP - 363
EP - 378
JO - Mycologia
JF - Mycologia
SN - 0027-5514
IS - 3
ER -