Biliary excretion of [(GS)2AsSe]- after intravenous injection of rabbits with arsenite and selenate

Jürgen Gailer, Graham N. George, Ingrid J. Pickering, Roger C. Prince, Husam S. Younis, Joy Winzerling

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

It has been shown that the seleno-bis (S-glutathionyl) arsinium ion, [(GS)2AsSe]-, is the major arsenic and selenium excretory product in bile of rabbits treated with arsenite and selenite [Gailer, J., Madden, S., Buttigieg, G. A., Denton, M. B., and Younis, H. S. (2002) Appl. Organomet. Chem. 16, 72-75]. To investigate the in vivo interaction between the other environmentally common oxy-anions of arsenic and selenium in mammals, we have intravenously injected rabbits with different combinations of the arsenic and selenium oxo-anions (arsenite + selenate, arsenate + selenite, and arsenate + selenate) and analyzed the collected bile and whole blood samples by X-ray absorption spectroscopy. Only the injection of arsenite and selenate led to the biliary excretion of [(GS)2AsSe]- within 25 min. Whole blood collected from these animals (25 min postinjection) contained predominantly unchanged selenate, which suggests the presence of a mammalian selenate reductase in the liver. The lack of any significant biliary excretion of [(GS)2AsSe]- in the other treatment groups implies that arsenate was not reduced in the liver on the time scale of our experiments. The relevance of these results for the human toxicology of arsenic and selenium is discussed.

Original languageEnglish (US)
Pages (from-to)1466-1471
Number of pages6
JournalChemical Research in Toxicology
Volume15
Issue number11
DOIs
StatePublished - Nov 1 2002

Fingerprint

Selenic Acid
Arsenic
Selenium
Intravenous Injections
Rabbits
Selenious Acid
Bile
Liver
Anions
Blood
X-Ray Absorption Spectroscopy
X ray absorption spectroscopy
Mammals
Toxicology
Animals
Ions
Injections
Hepatobiliary Elimination
arsenite
arsenic acid

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Biliary excretion of [(GS)2AsSe]- after intravenous injection of rabbits with arsenite and selenate. / Gailer, Jürgen; George, Graham N.; Pickering, Ingrid J.; Prince, Roger C.; Younis, Husam S.; Winzerling, Joy.

In: Chemical Research in Toxicology, Vol. 15, No. 11, 01.11.2002, p. 1466-1471.

Research output: Contribution to journalArticle

Gailer, Jürgen ; George, Graham N. ; Pickering, Ingrid J. ; Prince, Roger C. ; Younis, Husam S. ; Winzerling, Joy. / Biliary excretion of [(GS)2AsSe]- after intravenous injection of rabbits with arsenite and selenate. In: Chemical Research in Toxicology. 2002 ; Vol. 15, No. 11. pp. 1466-1471.
@article{5aa2eaca61aa4f14b79617b57b7eac6b,
title = "Biliary excretion of [(GS)2AsSe]- after intravenous injection of rabbits with arsenite and selenate",
abstract = "It has been shown that the seleno-bis (S-glutathionyl) arsinium ion, [(GS)2AsSe]-, is the major arsenic and selenium excretory product in bile of rabbits treated with arsenite and selenite [Gailer, J., Madden, S., Buttigieg, G. A., Denton, M. B., and Younis, H. S. (2002) Appl. Organomet. Chem. 16, 72-75]. To investigate the in vivo interaction between the other environmentally common oxy-anions of arsenic and selenium in mammals, we have intravenously injected rabbits with different combinations of the arsenic and selenium oxo-anions (arsenite + selenate, arsenate + selenite, and arsenate + selenate) and analyzed the collected bile and whole blood samples by X-ray absorption spectroscopy. Only the injection of arsenite and selenate led to the biliary excretion of [(GS)2AsSe]- within 25 min. Whole blood collected from these animals (25 min postinjection) contained predominantly unchanged selenate, which suggests the presence of a mammalian selenate reductase in the liver. The lack of any significant biliary excretion of [(GS)2AsSe]- in the other treatment groups implies that arsenate was not reduced in the liver on the time scale of our experiments. The relevance of these results for the human toxicology of arsenic and selenium is discussed.",
author = "J{\"u}rgen Gailer and George, {Graham N.} and Pickering, {Ingrid J.} and Prince, {Roger C.} and Younis, {Husam S.} and Joy Winzerling",
year = "2002",
month = "11",
day = "1",
doi = "10.1021/tx025538s",
language = "English (US)",
volume = "15",
pages = "1466--1471",
journal = "Chemical Research in Toxicology",
issn = "0893-228X",
publisher = "American Chemical Society",
number = "11",

}

TY - JOUR

T1 - Biliary excretion of [(GS)2AsSe]- after intravenous injection of rabbits with arsenite and selenate

AU - Gailer, Jürgen

AU - George, Graham N.

AU - Pickering, Ingrid J.

AU - Prince, Roger C.

AU - Younis, Husam S.

AU - Winzerling, Joy

PY - 2002/11/1

Y1 - 2002/11/1

N2 - It has been shown that the seleno-bis (S-glutathionyl) arsinium ion, [(GS)2AsSe]-, is the major arsenic and selenium excretory product in bile of rabbits treated with arsenite and selenite [Gailer, J., Madden, S., Buttigieg, G. A., Denton, M. B., and Younis, H. S. (2002) Appl. Organomet. Chem. 16, 72-75]. To investigate the in vivo interaction between the other environmentally common oxy-anions of arsenic and selenium in mammals, we have intravenously injected rabbits with different combinations of the arsenic and selenium oxo-anions (arsenite + selenate, arsenate + selenite, and arsenate + selenate) and analyzed the collected bile and whole blood samples by X-ray absorption spectroscopy. Only the injection of arsenite and selenate led to the biliary excretion of [(GS)2AsSe]- within 25 min. Whole blood collected from these animals (25 min postinjection) contained predominantly unchanged selenate, which suggests the presence of a mammalian selenate reductase in the liver. The lack of any significant biliary excretion of [(GS)2AsSe]- in the other treatment groups implies that arsenate was not reduced in the liver on the time scale of our experiments. The relevance of these results for the human toxicology of arsenic and selenium is discussed.

AB - It has been shown that the seleno-bis (S-glutathionyl) arsinium ion, [(GS)2AsSe]-, is the major arsenic and selenium excretory product in bile of rabbits treated with arsenite and selenite [Gailer, J., Madden, S., Buttigieg, G. A., Denton, M. B., and Younis, H. S. (2002) Appl. Organomet. Chem. 16, 72-75]. To investigate the in vivo interaction between the other environmentally common oxy-anions of arsenic and selenium in mammals, we have intravenously injected rabbits with different combinations of the arsenic and selenium oxo-anions (arsenite + selenate, arsenate + selenite, and arsenate + selenate) and analyzed the collected bile and whole blood samples by X-ray absorption spectroscopy. Only the injection of arsenite and selenate led to the biliary excretion of [(GS)2AsSe]- within 25 min. Whole blood collected from these animals (25 min postinjection) contained predominantly unchanged selenate, which suggests the presence of a mammalian selenate reductase in the liver. The lack of any significant biliary excretion of [(GS)2AsSe]- in the other treatment groups implies that arsenate was not reduced in the liver on the time scale of our experiments. The relevance of these results for the human toxicology of arsenic and selenium is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0036852790&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036852790&partnerID=8YFLogxK

U2 - 10.1021/tx025538s

DO - 10.1021/tx025538s

M3 - Article

C2 - 12437338

AN - SCOPUS:0036852790

VL - 15

SP - 1466

EP - 1471

JO - Chemical Research in Toxicology

JF - Chemical Research in Toxicology

SN - 0893-228X

IS - 11

ER -