Binding of Bacillus thuringiensis toxin Cry1Ac to multiple sites of cadherin in pink bollworm

J. A. Fabrick, Bruce E Tabashnik

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Toxins from Bacillus thuringiensis (Bt) are widely used for pest control. In particular, Bt toxin Cry1Ac produced by transgenic cotton kills some key lepidopteran pests. We found that Cry1Ac binds to recombinant peptides corresponding to extracellular regions of a cadherin protein (BtR) in a major cotton pest, pink bollworm (Pectinophora gossypiella) (PBW). In conjunction with previous results showing that PBW resistance to Cry1Ac is linked with mutations in the BtR gene, the results reported here support the hypothesis that BtR is a receptor for Cry1Ac in PBW. Similar to other lepidopteran cadherins that bind Bt toxins, BtR has at least two Cry1Ac-binding domains in cadherin-repeat regions 10 and 11, which are immediately adjacent to the membrane proximal region. However, unlike cadherins from Manduca sexta and Bombyx mori, toxin binding was not seen in regions more distal from the membrane proximal region. We also found that both the protoxin and activated toxin forms of Cry1Ac bound to recombinant BtR fragments, suggesting that Cry1Ac activation may occur either before or after receptor binding.

Original languageEnglish (US)
Pages (from-to)97-106
Number of pages10
JournalInsect Biochemistry and Molecular Biology
Volume37
Issue number2
DOIs
StatePublished - Feb 2007

Fingerprint

Pectinophora gossypiella
Bacillus thuringiensis
cadherins
Bacilli
Cadherins
toxins
Cotton
Pest control
Membranes
Manduca
cotton
Pest Control
Lepidoptera
Bombyx
pests
receptors
Manduca sexta
Bombyx mori
pest control
Genes

Keywords

  • Bacillus thuringiensis (Bt)
  • BtR
  • Cadherin
  • Cry1Ac receptor
  • Pectinophora gossypiella
  • Pink bollworm

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

Cite this

Binding of Bacillus thuringiensis toxin Cry1Ac to multiple sites of cadherin in pink bollworm. / Fabrick, J. A.; Tabashnik, Bruce E.

In: Insect Biochemistry and Molecular Biology, Vol. 37, No. 2, 02.2007, p. 97-106.

Research output: Contribution to journalArticle

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