Binding of PF2 lectin from Olneya tesota to gut proteins of Zabrotes subfasciatus larvae associated with the insecticidal mechanism

Irlanda Lagarda-Diaz, M. Refugio Robles-Burgeño, Ana M. Guzman-Partida, Dawn Geiser, Joy Winzerling, Luz Vazquez-Moreno

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Zabrotes subfasciatus (Boheman) is the main pest of common beans (Phaselous vulgaris). Wild legume seeds from Olneya tesota contain a lectin, PF2, that shows insecticidal activities against this insect. The binding of PF2 to midgut glycoproteins of 20-day-old larvae was evaluated using PF2 affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the proteins retained on the gel revealed several putative glycoproteins, ranging in mass from 17 to 97 kDa. Subsequent protein digestion and analysis by liquid chromatography-tandem mass spectrometry (LC-MS/ MS) provided amino acid fragments that identified an α-tubulin, cytochrome c oxidase subunit I, an odorant receptor, and a lysozyme from available insect sequence databases. The potential of these proteins to serve as part of the mechanisms involved in the insecticidal activity of PF2 to Z. subfasciatus is discussed.

Original languageEnglish (US)
Pages (from-to)2398-2402
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number9
DOIs
StatePublished - Mar 7 2012

Keywords

  • Affinity chromatography
  • Insect midgut glycoproteins
  • Olneya tesota
  • PF2 lectin
  • Wild legume
  • Zabrotes subfasciatus

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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