Biochemical and developmental studies of acetylcholine metabolism in the central nervous system of the moth Manduca sexta

D. J. Prescott, John G Hildebrand, J. R. Sanes, S. Jewett

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

1. Acetylcholine and its biosynthetic and degradative enzymes (choline acetyltransferase and acetylcholinesterase, respectively) were quantified in several defined structures from the central nervous system of the tobacco hornworm Manduca sexta at various times during postembryonic development. 2. Activity of choline acetyltransferase, the key enzyme of the cholinergic pathway, changes roughly synchronously and similarly in the protocerebrum and subesophageal, prothoracic, and third abdominal ganglia, and it increases dramatically in developing antennal and optic lobes. 3. Levels of acetylcholine and of specific acetylcholinesterase, which is the principal cholinesterase in the nervous system, also rise greatly during metamorphosis of the central nervous regions. 4. The major changes in the activity of choline acetyltransferase correlate with known developmental endocrine events.

Original languageEnglish (US)
Pages (from-to)77-84
Number of pages8
JournalComparative Biochemistry and Physiology. Part C, Comparative
Volume56
Issue number2
DOIs
StatePublished - 1977
Externally publishedYes

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Manduca
Choline O-Acetyltransferase
Moths
Acetylcholine
Central Nervous System
Acetylcholinesterase
Cholinesterases
Enzymes
Ganglia
Cholinergic Agents
Nervous System

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "1. Acetylcholine and its biosynthetic and degradative enzymes (choline acetyltransferase and acetylcholinesterase, respectively) were quantified in several defined structures from the central nervous system of the tobacco hornworm Manduca sexta at various times during postembryonic development. 2. Activity of choline acetyltransferase, the key enzyme of the cholinergic pathway, changes roughly synchronously and similarly in the protocerebrum and subesophageal, prothoracic, and third abdominal ganglia, and it increases dramatically in developing antennal and optic lobes. 3. Levels of acetylcholine and of specific acetylcholinesterase, which is the principal cholinesterase in the nervous system, also rise greatly during metamorphosis of the central nervous regions. 4. The major changes in the activity of choline acetyltransferase correlate with known developmental endocrine events.",
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T1 - Biochemical and developmental studies of acetylcholine metabolism in the central nervous system of the moth Manduca sexta

AU - Prescott, D. J.

AU - Hildebrand, John G

AU - Sanes, J. R.

AU - Jewett, S.

PY - 1977

Y1 - 1977

N2 - 1. Acetylcholine and its biosynthetic and degradative enzymes (choline acetyltransferase and acetylcholinesterase, respectively) were quantified in several defined structures from the central nervous system of the tobacco hornworm Manduca sexta at various times during postembryonic development. 2. Activity of choline acetyltransferase, the key enzyme of the cholinergic pathway, changes roughly synchronously and similarly in the protocerebrum and subesophageal, prothoracic, and third abdominal ganglia, and it increases dramatically in developing antennal and optic lobes. 3. Levels of acetylcholine and of specific acetylcholinesterase, which is the principal cholinesterase in the nervous system, also rise greatly during metamorphosis of the central nervous regions. 4. The major changes in the activity of choline acetyltransferase correlate with known developmental endocrine events.

AB - 1. Acetylcholine and its biosynthetic and degradative enzymes (choline acetyltransferase and acetylcholinesterase, respectively) were quantified in several defined structures from the central nervous system of the tobacco hornworm Manduca sexta at various times during postembryonic development. 2. Activity of choline acetyltransferase, the key enzyme of the cholinergic pathway, changes roughly synchronously and similarly in the protocerebrum and subesophageal, prothoracic, and third abdominal ganglia, and it increases dramatically in developing antennal and optic lobes. 3. Levels of acetylcholine and of specific acetylcholinesterase, which is the principal cholinesterase in the nervous system, also rise greatly during metamorphosis of the central nervous regions. 4. The major changes in the activity of choline acetyltransferase correlate with known developmental endocrine events.

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