Biochemical and Spectroscopic Characterization of a Radical S-Adenosyl- l -methionine Enzyme Involved in the Formation of a Peptide Thioether Cross-Link

Nathan A. Bruender, Jarett Wilcoxen, R. David Britt, Vahe Bandarian

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Peptide-derived natural products are a class of metabolites that afford the producing organism a selective advantage over other organisms in their biological niche. While the polypeptide antibiotics produced by the nonribosomal polypeptide synthetases (NRPS) are the most widely recognized, the ribosomally synthesized and post-translationally modified peptides (RiPPs) are an emerging group of natural products with diverse structures and biological functions. Both the NRPS derived peptides and the RiPPs undergo extensive post-translational modifications to produce structural diversity. Here we report the first characterization of the six cysteines in forty-five (SCIFF) [Haft, D. H. and Basu M. K. (2011) J. Bacteriol. 193, 2745-2755] peptide maturase Tte1186, which is a member of the radical S-adenosyl-l-methionine (SAM) superfamily. Tte1186 catalyzes the formation of a thioether cross-link in the peptide Tte1186a encoded by an orf located upstream of the maturase, under reducing conditions in the presence of SAM. Tte1186 contains three [4Fe-4S] clusters that are indispensable for thioether cross-link formation; however, only one cluster catalyzes the reductive cleavage of SAM. Mechanistic imperatives for the reaction catalyzed by the thioether forming radical SAM maturases will be discussed.

Original languageEnglish (US)
Pages (from-to)2122-2134
Number of pages13
JournalBiochemistry
Volume55
Issue number14
DOIs
StatePublished - Apr 26 2016
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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