Biochemical, genetic and physiological characterization of venom components from two species of scorpions: Centruroides exilicauda Wood and Centruroides sculpturatus Ewing

Norma A. Valdez-Cruz, Sonia Dávila, Alexei Licea, Miguel Corona, Fernando Z. Zamudio, Jesús García-Valdes, Leslie V Boyer, Lourival D. Possani

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Current literature concerning the taxonomic names of two possibly distinct species of scorpions from the genus Centruroides (sculpturatus and/or exilicauda) is controversial. This communication reports the results of biochemical, genetic and electrophysiological experiments conducted with C. exilicauda Wood of Baja California (Mexico) and C. sculpturatus Ewing of Arizona (USA). The chromatographic profile fractionation of the soluble venom from both species of scorpions is different. The N-terminal amino acid sequence for nine toxins of C. exilicauda was determined and compared with those from C. sculpturatus. Lethality tests conducted in mice support the idea that C. exilicauda venom should be expected to be medically less important than C. sculpturatus. Thirteen genes from the venomous glands of the scorpion C. exilicauda were obtained and compared with previously published sequences from genes of the species C. sculpturatus. Genes coding for cytochrome oxidase I and II of both species were also sequenced. A phylogenetic tree was generated with this information showing important differences between them. Additionally, the results of electrophysiological assays conducted with the venom from both species on the Ca 2+-dependent K +-channels, showed significant differences. These results strongly support the conclusion that C. exilicauda and C. sculpturatus are in fact two distinct species of scorpions.

Original languageEnglish (US)
Pages (from-to)387-396
Number of pages10
JournalBiochimie
Volume86
Issue number6
DOIs
StatePublished - Jun 2004

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Scorpions
Venoms
Molecular Biology
Wood
Genes
Electron Transport Complex IV
Fractionation
Assays
Mexico
Names
Amino Acids
Amino Acid Sequence
Communication
Experiments

Keywords

  • C
  • C. exilicauda
  • C. sculpturatus
  • Centruroides
  • COX I
  • COX II
  • Cytochrome oxidase
  • cytochrome oxidase I
  • cytochrome oxidase II
  • K -channel
  • l
  • limpidus
  • mDNA
  • mitochondrial DNA
  • Mitochondrial gene
  • Scorpion toxin
  • T
  • Tityus

ASJC Scopus subject areas

  • Biochemistry

Cite this

Biochemical, genetic and physiological characterization of venom components from two species of scorpions : Centruroides exilicauda Wood and Centruroides sculpturatus Ewing. / Valdez-Cruz, Norma A.; Dávila, Sonia; Licea, Alexei; Corona, Miguel; Zamudio, Fernando Z.; García-Valdes, Jesús; Boyer, Leslie V; Possani, Lourival D.

In: Biochimie, Vol. 86, No. 6, 06.2004, p. 387-396.

Research output: Contribution to journalArticle

Valdez-Cruz, Norma A. ; Dávila, Sonia ; Licea, Alexei ; Corona, Miguel ; Zamudio, Fernando Z. ; García-Valdes, Jesús ; Boyer, Leslie V ; Possani, Lourival D. / Biochemical, genetic and physiological characterization of venom components from two species of scorpions : Centruroides exilicauda Wood and Centruroides sculpturatus Ewing. In: Biochimie. 2004 ; Vol. 86, No. 6. pp. 387-396.
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