Biosensor technology includes the detection of generated biological functionality. One whole-cell biosensor concept leaves specific receptor molecules in their natural environment to be optimized and adapted for efficient molecular recognition during evolution, and to use intact cells as biologically-active elements. In this project, a basic nanomaterials platform for whole cell sensors is produced by electrospinning composite nano- and microfiber networks. The resulting polymer-metal oxide (titania) "nano-mats" are fabricated on a conductive indium tin oxide (ITO) coated glass substrate for epithelial or other cell incubation and future incorporation into bioelectronics. Impedance spectroscopy is used to characterize cell interactions with the nano-mat to detect changes in cells with proliferation or when exposed to chemical stimuli, such as lactic acid. Cell response to media conditions can include growth, attachment and s preading, motility and migration, and cell death.