Biodegradability of N-substituted aromatics and alkylphenols under methanogenic conditions using granular sludge

Elías Razo-Flores, Brian Donlon, James A Field, Gatze Lettinga

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The biodegradability of seventeen N-substituted aromatic and six alkylphenol compounds were evaluated under methanogenic conditions. Biodegradation was assessed in batch assays inoculated with unacclimated and predigested anaerobic granular sludge at 30°C under agitated conditions over a 150 day period. The compounds were supplied at sub-toxic concentrations in the assays in order to prevent inhibition to the methanogens. The biodegradability test was performed by the measurement of the methane composition in the headspace of the serum flasks. The methanogenic consortia completely mineralized 2-, 3-aminobenzoate, 2-aminophenol and 4-cresol; whereas, 4-aminobenzoate was only partially degraded. The other N-substituted compounds and the alkylphenols tested were not biodegradable under the experimental conditions employed. An additional biodegradability assay was conducted with sludge from an upward-flow anaerobic sludge bed reactor adapted to the degradation of 2-nitrophenol. This sludge mineralized 2-aminophenol without any lag phase while the unadapted sludge required 110 days of acclimation. The three aminobenzoate isomers were fully mineralized by the adapted sludge after similar lag periods observed in the unadapted sludge. The 2-nitrophenol adapted sludge cross-acclimatized to the mineralization of 5-aminosalicylate and 4-aminophenol. This constitutes the first report demonstrating the anaerobic mineralization of 5-aminosalicylate, which indicates that at least some azo dye cleavage products can be degraded in methanogenic consortia.

Original languageEnglish (US)
Pages (from-to)47-57
Number of pages11
JournalWater Science and Technology
Volume33
Issue number3
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Biodegradability
Assays
sludge
Methanogens
Azo dyes
Biodegradation
Isomers
Methane
assay
Degradation
mineralization
Chemical analysis
biodegradability
acclimation
cleavage
serum
biodegradation
dye
Nitrophenols
methane

Keywords

  • 5-aminosalicylate
  • Alkylphenols
  • Aminobenzoate
  • Aminophenol
  • Anaerobic degradation
  • Azo dye cleavage products
  • Granular sludge
  • Methanogenic conditions
  • N-substituted aromatics

ASJC Scopus subject areas

  • Water Science and Technology

Cite this

Biodegradability of N-substituted aromatics and alkylphenols under methanogenic conditions using granular sludge. / Razo-Flores, Elías; Donlon, Brian; Field, James A; Lettinga, Gatze.

In: Water Science and Technology, Vol. 33, No. 3, 1996, p. 47-57.

Research output: Contribution to journalArticle

@article{fa0bfd119cd4408296a86bb7d16467c4,
title = "Biodegradability of N-substituted aromatics and alkylphenols under methanogenic conditions using granular sludge",
abstract = "The biodegradability of seventeen N-substituted aromatic and six alkylphenol compounds were evaluated under methanogenic conditions. Biodegradation was assessed in batch assays inoculated with unacclimated and predigested anaerobic granular sludge at 30°C under agitated conditions over a 150 day period. The compounds were supplied at sub-toxic concentrations in the assays in order to prevent inhibition to the methanogens. The biodegradability test was performed by the measurement of the methane composition in the headspace of the serum flasks. The methanogenic consortia completely mineralized 2-, 3-aminobenzoate, 2-aminophenol and 4-cresol; whereas, 4-aminobenzoate was only partially degraded. The other N-substituted compounds and the alkylphenols tested were not biodegradable under the experimental conditions employed. An additional biodegradability assay was conducted with sludge from an upward-flow anaerobic sludge bed reactor adapted to the degradation of 2-nitrophenol. This sludge mineralized 2-aminophenol without any lag phase while the unadapted sludge required 110 days of acclimation. The three aminobenzoate isomers were fully mineralized by the adapted sludge after similar lag periods observed in the unadapted sludge. The 2-nitrophenol adapted sludge cross-acclimatized to the mineralization of 5-aminosalicylate and 4-aminophenol. This constitutes the first report demonstrating the anaerobic mineralization of 5-aminosalicylate, which indicates that at least some azo dye cleavage products can be degraded in methanogenic consortia.",
keywords = "5-aminosalicylate, Alkylphenols, Aminobenzoate, Aminophenol, Anaerobic degradation, Azo dye cleavage products, Granular sludge, Methanogenic conditions, N-substituted aromatics",
author = "El{\'i}as Razo-Flores and Brian Donlon and Field, {James A} and Gatze Lettinga",
year = "1996",
doi = "10.1016/0273-1223(96)00320-4",
language = "English (US)",
volume = "33",
pages = "47--57",
journal = "Water Science and Technology",
issn = "0273-1223",
publisher = "IWA Publishing",
number = "3",

}

TY - JOUR

T1 - Biodegradability of N-substituted aromatics and alkylphenols under methanogenic conditions using granular sludge

AU - Razo-Flores, Elías

AU - Donlon, Brian

AU - Field, James A

AU - Lettinga, Gatze

PY - 1996

Y1 - 1996

N2 - The biodegradability of seventeen N-substituted aromatic and six alkylphenol compounds were evaluated under methanogenic conditions. Biodegradation was assessed in batch assays inoculated with unacclimated and predigested anaerobic granular sludge at 30°C under agitated conditions over a 150 day period. The compounds were supplied at sub-toxic concentrations in the assays in order to prevent inhibition to the methanogens. The biodegradability test was performed by the measurement of the methane composition in the headspace of the serum flasks. The methanogenic consortia completely mineralized 2-, 3-aminobenzoate, 2-aminophenol and 4-cresol; whereas, 4-aminobenzoate was only partially degraded. The other N-substituted compounds and the alkylphenols tested were not biodegradable under the experimental conditions employed. An additional biodegradability assay was conducted with sludge from an upward-flow anaerobic sludge bed reactor adapted to the degradation of 2-nitrophenol. This sludge mineralized 2-aminophenol without any lag phase while the unadapted sludge required 110 days of acclimation. The three aminobenzoate isomers were fully mineralized by the adapted sludge after similar lag periods observed in the unadapted sludge. The 2-nitrophenol adapted sludge cross-acclimatized to the mineralization of 5-aminosalicylate and 4-aminophenol. This constitutes the first report demonstrating the anaerobic mineralization of 5-aminosalicylate, which indicates that at least some azo dye cleavage products can be degraded in methanogenic consortia.

AB - The biodegradability of seventeen N-substituted aromatic and six alkylphenol compounds were evaluated under methanogenic conditions. Biodegradation was assessed in batch assays inoculated with unacclimated and predigested anaerobic granular sludge at 30°C under agitated conditions over a 150 day period. The compounds were supplied at sub-toxic concentrations in the assays in order to prevent inhibition to the methanogens. The biodegradability test was performed by the measurement of the methane composition in the headspace of the serum flasks. The methanogenic consortia completely mineralized 2-, 3-aminobenzoate, 2-aminophenol and 4-cresol; whereas, 4-aminobenzoate was only partially degraded. The other N-substituted compounds and the alkylphenols tested were not biodegradable under the experimental conditions employed. An additional biodegradability assay was conducted with sludge from an upward-flow anaerobic sludge bed reactor adapted to the degradation of 2-nitrophenol. This sludge mineralized 2-aminophenol without any lag phase while the unadapted sludge required 110 days of acclimation. The three aminobenzoate isomers were fully mineralized by the adapted sludge after similar lag periods observed in the unadapted sludge. The 2-nitrophenol adapted sludge cross-acclimatized to the mineralization of 5-aminosalicylate and 4-aminophenol. This constitutes the first report demonstrating the anaerobic mineralization of 5-aminosalicylate, which indicates that at least some azo dye cleavage products can be degraded in methanogenic consortia.

KW - 5-aminosalicylate

KW - Alkylphenols

KW - Aminobenzoate

KW - Aminophenol

KW - Anaerobic degradation

KW - Azo dye cleavage products

KW - Granular sludge

KW - Methanogenic conditions

KW - N-substituted aromatics

UR - http://www.scopus.com/inward/record.url?scp=0029952241&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029952241&partnerID=8YFLogxK

U2 - 10.1016/0273-1223(96)00320-4

DO - 10.1016/0273-1223(96)00320-4

M3 - Article

AN - SCOPUS:0029952241

VL - 33

SP - 47

EP - 57

JO - Water Science and Technology

JF - Water Science and Technology

SN - 0273-1223

IS - 3

ER -