Abstract
The biodegradability of seventeen N-substituted aromatic and six alkylphenol compounds were evaluated under methanogenic conditions. Biodegradation was assessed in batch assays inoculated with unacclimated and predigested anaerobic granular sludge at 30°C under agitated conditions over a 150 day period. The compounds were supplied at sub-toxic concentrations in the assays in order to prevent inhibition to the methanogens. The biodegradability test was performed by the measurement of the methane composition in the headspace of the serum flasks. The methanogenic consortia completely mineralized 2-, 3-aminobenzoate, 2-aminophenol and 4-cresol; whereas, 4-aminobenzoate was only partially degraded. The other N-substituted compounds and the alkylphenols tested were not biodegradable under the experimental conditions employed. An additional biodegradability assay was conducted with sludge from an upward-flow anaerobic sludge bed reactor adapted to the degradation of 2-nitrophenol. This sludge mineralized 2-aminophenol without any lag phase while the unadapted sludge required 110 days of acclimation. The three aminobenzoate isomers were fully mineralized by the adapted sludge after similar lag periods observed in the unadapted sludge. The 2-nitrophenol adapted sludge cross-acclimatized to the mineralization of 5-aminosalicylate and 4-aminophenol. This constitutes the first report demonstrating the anaerobic mineralization of 5-aminosalicylate, which indicates that at least some azo dye cleavage products can be degraded in methanogenic consortia.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 47-57 |
| Number of pages | 11 |
| Journal | Water Science and Technology |
| Volume | 33 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1996 |
| Externally published | Yes |
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Keywords
- 5-aminosalicylate
- Alkylphenols
- Aminobenzoate
- Aminophenol
- Anaerobic degradation
- Azo dye cleavage products
- Granular sludge
- Methanogenic conditions
- N-substituted aromatics
ASJC Scopus subject areas
- Water Science and Technology
Cite this
Biodegradability of N-substituted aromatics and alkylphenols under methanogenic conditions using granular sludge. / Razo-Flores, Elías; Donlon, Brian; Field, James A; Lettinga, Gatze.
In: Water Science and Technology, Vol. 33, No. 3, 1996, p. 47-57.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biodegradability of N-substituted aromatics and alkylphenols under methanogenic conditions using granular sludge
AU - Razo-Flores, Elías
AU - Donlon, Brian
AU - Field, James A
AU - Lettinga, Gatze
PY - 1996
Y1 - 1996
N2 - The biodegradability of seventeen N-substituted aromatic and six alkylphenol compounds were evaluated under methanogenic conditions. Biodegradation was assessed in batch assays inoculated with unacclimated and predigested anaerobic granular sludge at 30°C under agitated conditions over a 150 day period. The compounds were supplied at sub-toxic concentrations in the assays in order to prevent inhibition to the methanogens. The biodegradability test was performed by the measurement of the methane composition in the headspace of the serum flasks. The methanogenic consortia completely mineralized 2-, 3-aminobenzoate, 2-aminophenol and 4-cresol; whereas, 4-aminobenzoate was only partially degraded. The other N-substituted compounds and the alkylphenols tested were not biodegradable under the experimental conditions employed. An additional biodegradability assay was conducted with sludge from an upward-flow anaerobic sludge bed reactor adapted to the degradation of 2-nitrophenol. This sludge mineralized 2-aminophenol without any lag phase while the unadapted sludge required 110 days of acclimation. The three aminobenzoate isomers were fully mineralized by the adapted sludge after similar lag periods observed in the unadapted sludge. The 2-nitrophenol adapted sludge cross-acclimatized to the mineralization of 5-aminosalicylate and 4-aminophenol. This constitutes the first report demonstrating the anaerobic mineralization of 5-aminosalicylate, which indicates that at least some azo dye cleavage products can be degraded in methanogenic consortia.
AB - The biodegradability of seventeen N-substituted aromatic and six alkylphenol compounds were evaluated under methanogenic conditions. Biodegradation was assessed in batch assays inoculated with unacclimated and predigested anaerobic granular sludge at 30°C under agitated conditions over a 150 day period. The compounds were supplied at sub-toxic concentrations in the assays in order to prevent inhibition to the methanogens. The biodegradability test was performed by the measurement of the methane composition in the headspace of the serum flasks. The methanogenic consortia completely mineralized 2-, 3-aminobenzoate, 2-aminophenol and 4-cresol; whereas, 4-aminobenzoate was only partially degraded. The other N-substituted compounds and the alkylphenols tested were not biodegradable under the experimental conditions employed. An additional biodegradability assay was conducted with sludge from an upward-flow anaerobic sludge bed reactor adapted to the degradation of 2-nitrophenol. This sludge mineralized 2-aminophenol without any lag phase while the unadapted sludge required 110 days of acclimation. The three aminobenzoate isomers were fully mineralized by the adapted sludge after similar lag periods observed in the unadapted sludge. The 2-nitrophenol adapted sludge cross-acclimatized to the mineralization of 5-aminosalicylate and 4-aminophenol. This constitutes the first report demonstrating the anaerobic mineralization of 5-aminosalicylate, which indicates that at least some azo dye cleavage products can be degraded in methanogenic consortia.
KW - 5-aminosalicylate
KW - Alkylphenols
KW - Aminobenzoate
KW - Aminophenol
KW - Anaerobic degradation
KW - Azo dye cleavage products
KW - Granular sludge
KW - Methanogenic conditions
KW - N-substituted aromatics
UR - http://www.scopus.com/inward/record.url?scp=0029952241&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029952241&partnerID=8YFLogxK
U2 - 10.1016/0273-1223(96)00320-4
DO - 10.1016/0273-1223(96)00320-4
M3 - Article
AN - SCOPUS:0029952241
VL - 33
SP - 47
EP - 57
JO - Water Science and Technology
JF - Water Science and Technology
SN - 0273-1223
IS - 3
ER -